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pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

Mouse Pgk1

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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HUWE1-sgRNA-1

Plasmid

#86924

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA1 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
HUWE1-sgRNA-2

Plasmid

#86925

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA2 (HUWE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only
pMGIB-3xFLAG-pontin

Plasmid

#53608

Purpose

Retroviral expression of human pontin

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

pontin (RUVBL1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

pontin CDS mutated to confer resistance to shRNA.…

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pCRISPReporter-mCherry

Plasmid

#65008

Purpose

E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Insert

mCherry

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

User-defined

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL-gRNA(anti-cGAS)-Cas9-Puro

Plasmid

#130908

Purpose

gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Cas9

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dnmt3b-L

Plasmid

#122334

Purpose

Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dnmt3b

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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Mfn1(K88T)-10xmyc

Plasmid

#26050

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Tags

10xMyc

Expression

Mammalian

Mutation

K88T GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
mCherry-p62-LIR-mutant (LIRAAAA)

Plasmid

#187981

Purpose

Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DDDW335-338AAAA (LIR mutant), silent mutation siR…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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Flag Intersectin Long (human)

Plasmid

#47393

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-Sema4D-Flag

Plasmid

#190646

Purpose

Expresses Mouse Sema4D with C Terminal Flag Tag

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4d (Sema4d Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-DEPTOR

Plasmid

#73390

Purpose

Expresses HA and YFP tagged DEPTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
YFP-DAXX-mSIM1/2

Plasmid

#134525

Purpose

Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1/2

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG and SIM (IIVL)…

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST15 hSlp4-a

Plasmid

#40046

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a (SYTL4 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
hP2X3-cALFA

Plasmid

#160499

Purpose

For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

human P2X3 (P2RX3 Human, Synthetic)

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

ΔN5ΔC33-T13P/S15V/V16I

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_936

Plasmid

#216094

Purpose

Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD46, CD47, CD55, CD81 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Tags

HA

Expression

Mammalian

Mutation

E186Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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