We narrowed to 23,858 results for: ESC

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pSW002-Pc-DsRed-Express2

Plasmid

#111251

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-mCherry

Plasmid

#65253

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-Cre

Plasmid

#50935

Purpose

retroviral expression of Cre recombinase

Depositor

Pier Pandolfi

Article

Chen et al Nature. 2005 Aug 4;436(7051):725-30.

Insert

Cre recombinase

Use

Cre/Lox and Retroviral

Tags

NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN-sDarken

Plasmid

#184796

Purpose

Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

sDarken

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209656

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pREDKI

Plasmid

#51626

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

pRha

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Mito-7

Plasmid

#58023

Purpose

Localization: Mitochondria, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Mito (COX8A Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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FSW-ALDH2-E504K-T2A-EGFP

Plasmid

#197037

Purpose

Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2-E504K

Use

Lentiviral

Expression

Mammalian

Mutation

E504K

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-Golgi-7

Plasmid

#58100

Purpose

Localization: Golgi, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Golgi (B4GALT1 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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FL-mCherry

Plasmid

#78862

Purpose

DULIP negative control (prey) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCD86-mCherry2

Plasmid

#190747

Purpose

Expression mCherry2-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

CD86 (CD86 Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PTP1B human

Plasmid

#102719

Purpose

human PTP1B catalytic domain (residues 1-301) in RP1B vector

Depositor

Wolfgang Peti

Article

Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

Insert

Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal no NLS

Plasmid

#188397

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

Phyper spank

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209664

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209665

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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