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APEX2-SOPP3-TM

Plasmid

#229077

Purpose

Expresses APEX2-SOPP3 in the cell surface

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the cell surface (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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8522-M31-663

Plasmid

#225661

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCherry

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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OTR untagged

Plasmid

#208913

Purpose

encodes the human oxytocin receptor, without any tags

Depositor

Christian Gruber

Article

Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

Insert

OXTR (OXTR Human)

Expression

Mammalian

Mutation

TM5: 5x56, A218, Threonin instead of Alanin

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00028 (aka pTS0057)

Plasmid

#200832

Purpose

AAVS1 guide RNA cloned into the lentiSAM puro backbone

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Insert

AAVS1 guide RNA

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A/S311A

Plasmid

#213589

Purpose

Doxycycline inducible expression of TAZ-S89A/S311A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A, S311A

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-BRD4ΔN

Plasmid

#200968

Purpose

induction of phase-separated condensate mediated by BRD4dN-IDR

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

BRD4dN

Expression

Bacterial and Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp12

Plasmid

#168949

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp12 (ORF1ab )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Nucleocapsid

Plasmid

#168906

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Nucleocapsid (N )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN-mut

Plasmid

#200970

Purpose

induction of phase-separated condensate mediated by FUSN-IDR mutant

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSNmut

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

15 Tyr-to-Ser substitutions

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust

Plasmid

#200969

Purpose

induction of phase-separated condensate by CRY2Clust

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

CRY2Clust

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET29_LuxSit_i

Plasmid

#194299

Purpose

Bacterial expression of the de novo luciferase, LuxSit-i

Depositor

David Baker, Andy Yeh

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Expression

Bacterial

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_EGFP

Plasmid

#179392

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

EGFP

Tags

AviTag

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:mCherry-PA-Rac1

Plasmid

#169044

Purpose

Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

PA-Rac-1 (RAC1 Human, Avena sativa (oat))

Tags

6X His and Fused with mCherry

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP6450

Plasmid

#128731

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

Insert

HopAD1

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pV2A-T

Plasmid

#153022

Purpose

Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

Depositor

Vito Valiante

Article

Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pASPIre3

Plasmid

#154844

Purpose

Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

Depositor

Markus Jeschek

Article

Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

Insert

Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator

Expression

Bacterial

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-shEsr2

Plasmid

#120722

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

Depositor

Amy Lasek

Article

Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

Insert

Esr2 (Esr2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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