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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-24xopto-TetO-no01

Plasmid

#174887

Purpose

Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

24xopto-TetO

Use

Southern blot probe

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF

Plasmid

#177267

Purpose

CRISPR vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-T#1

Plasmid

#171515

Purpose

deletion of a genomic locus in T(Brachyury) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T (T Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-T#2

Plasmid

#171516

Purpose

deletion of a genomic locus in T(Brachyury) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T (T Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L1-#1

Plasmid

#171503

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L1-#2

Plasmid

#171504

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L2-#1

Plasmid

#171505

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L2-#2

Plasmid

#171506

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-LexA-CIB1-biLINuS

Plasmid

#192607

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-eGFP-P2A-CRY2-VPR

Plasmid

#192610

Purpose

Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

eGFP-P2A-CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Tags

eGFP

Promoter

PGK

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.71

Plasmid

#184983

Purpose

Express -Eco1 RT/Cas9 fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker1-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-9S-S2 [rpoA]

Plasmid

#176217

Purpose

Expression of TRX-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

HIS and TRX

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-9P-S2 [rpoA]

Plasmid

#176218

Purpose

Expression of TRX-9P-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9P-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-9S-DYW [rpoA]

Plasmid

#176220

Purpose

Expression of TRX-9S-DYW [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-9S-DYW [rpoA]

Use

Synthetic Biology

Tags

TRX-His

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pETM20-avi-9S-S2 [rpoA]

Plasmid

#176223

Purpose

Expression of TRX-AviTag-9S-S2 [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

TRX-Avi-9S-S2 [rpoA]

Use

Synthetic Biology

Tags

TRX-His-AviTag

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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