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FUW-SM

Plasmid

#20330

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pPac Sp1

Plasmid

#12095

Depositor

Robert Tjian

Article

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Insert

Sp1 (SP1 Human)

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-HypaCas9

Plasmid

#108302

Purpose

pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAM1579

Plasmid

#40240

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Km Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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sfTq2ox-PBP5

Plasmid

#117960

Purpose

Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2ox-PBP5 (dacA )

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SLC38A9.1

Plasmid

#71869

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
mEmerald-Kinesin11-N-18

Plasmid

#54137

Purpose

Localization: Motor Protein/Microtubule Filaments, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Kinesin11 (KIF11 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mEGFP-Cdc42-C1

Plasmid

#29673

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

Cdc42 (CDC42 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pHes5p-luc

Plasmid

#26869

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

Hes5 promoter (Hes5 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only
pFA6a-6xGLY-His-tag-HIS3MX6

Plasmid

#20762

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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GZnP3 in pcDNA3.1+

Plasmid

#161738

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

Insert

GZnP3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3B

Plasmid

#137757

Purpose

Expression of LC3B

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-4x

Plasmid

#124678

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-ICUE

Plasmid

#118480

Purpose

ddRFP-based single-color biosensor for monitoring cAMP dynamics.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-ICUE

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pT2/SV-Venus-SIINFEKL

Plasmid

#111624

Purpose

Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

Depositor

Jon Yewdell

Insert

Venus-LEQLE-SIINFEKL-TEW

Tags

LEQLE-SIINFEKL-TEW

Expression

Mammalian

Promoter

SV40

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luc-C

Plasmid

#58791

Purpose

Express LucC alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 394-550

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only