We narrowed to 6,478 results for: nls

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TDP-43deltaNLS-V5-APEX

Plasmid

#229081

Purpose

Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone

Depositor

Eran Hornstein

Insert

TDP-43deltaNLS (TARDBP Human)

Tags

APEX, BFP, and V5

Expression

Mammalian

Mutation

K82A, R83A, K84A, K95A, K97A and R98A for mutatio…

Promoter

TRE3G

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-TagRFP-SV40

Plasmid

#117805

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-NLS-cTF

Plasmid

#87746

Purpose

IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and NLS linker - cTF (chimeric transcriptio…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-2xNLS-mTurquoise2

Plasmid

#118025

Purpose

An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Promoter

hSyn1

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pPBpuro-hM3D-P2Av3-mCherryNLS

Plasmid

#184714

Purpose

Gq DREADD

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

hM3D

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AID∆C-Cas9n-UGI-NLS

Plasmid

#198890

Purpose

Express AID(1-188) BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Actication induced deaminase (AICDA Human)

Tags

NLS

Expression

Mammalian

Mutation

Deletion of amino acids 189-198

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VPR

Plasmid

#183936

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

GFP

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-NS3-NLS/VPR

Plasmid

#112244

Purpose

Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

Depositor

John Ngo

Article

Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

Insert

dCas9-NS3-NLS/VPR

Tags

AU1 (N term on NS3) and HA (C term on NS3)

Expression

Mammalian

Mutation

NS3 mutant T54A

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-tdTomato-cNLS

Plasmid

#138532

Purpose

Lentiviral inducible expression of tdTomato with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

tdTomato

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE-CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-LacI-NLS-VP16

Plasmid

#103836

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-tagRFP-NLS-HA-Bxb1

Plasmid

#65625

Purpose

Vector for cotranslational expression of tagRFP-Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Bxb1 integrase

Tags

HA, SV40 NLS, and tagRFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHiFiNG-P2A-Puro

Plasmid

#136903

Purpose

Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHiFiNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-ICUE3-NLS

Plasmid

#138255

Purpose

FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

Depositor

Jin Zhang

Article

Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

Insert

FKBP-ICUE3-NLS

Tags

FKBP, Nuclear localization signal (NLS), cpVenus[…

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

Plasmid

#124705

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS-LL/RK-NLS/D307A

Plasmid

#185459

Purpose

Inducible nuclear cGAS with D307A mutation

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

L157R, L159K, D307A

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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