We narrowed to 4,308 results for: PRS

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pRS416 Gal-RNQ1-YFP

Plasmid

#18685

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pRS2 (HIF2a-pRetro-Super)

Plasmid

#22100

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1)

Plasmid

#58270

Purpose

bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pRS405 PEX14-VAC8-RFP

Plasmid

#166844

Purpose

Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, PEX14 Budding Yeast)

Tags

PEX14 transmembrane domain and RFP

Expression

Yeast

Mutation

PEX14 transmembrane domain

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-RFP

Plasmid

#166843

Purpose

Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Tags

RFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS9 (HIF2a-pRetro-Super)

Plasmid

#22101

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81D

Plasmid

#129351

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81D

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu E83A

Plasmid

#129353

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu E83A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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PRS_8-jGCaMP7b-P2A-mRuby3

Plasmid

#192595

Purpose

AAV-vector for red fluorescent labeling and green fluorescent calcium measurements from noradrenergic neurons

Depositor

Simon Wiegert

Insert

jGCaMP7b

Use

AAV

Tags

mRuby3

Expression

Mammalian

Promoter

PRSX8

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-triple mutation-pa-GCaMP6f

Plasmid

#67570

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

V115H, L64F, T65S

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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CT-Nano-lantern(ATP1)/pRSETB

Plasmid

#51990

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Tags

6xHis and chloroplast transit peptid

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pRS305:Met3p-mCherry-Tub1

Plasmid

#37553

Depositor

Wei-Lih Lee

Article

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

Met3p-mCherry-Tub1

Expression

Bacterial

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only
pRS406 ATG1-ATG17-3'UTR

Plasmid

#166848

Purpose

Expresses Atg1 with ATG17 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 G294A

Plasmid

#27459

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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