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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,850 results for: sequence

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Showing: 10201 - 10220 of 13850 results

  3. BMR1_02g00320-bio-His

    Plasmid
    #108028
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Insert
    BMR1_02g00320
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108028-plasmid-map-sequence-id-213147

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  4. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  7. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid
    #24417
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Insert
    Rs1
    Use
    Entry vector
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    short TetO, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24417-plasmid-map-sequence-id-11414

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  10. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid
    #176244
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Promoter
    Ribosomal subunit
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176244-plasmid-map-sequence-id-346573

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  12. pMTB2-RanGAP-Cerulean-Avi

    Plasmid
    #80068
    Purpose
    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Use
    Zebrafish biotagging
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    Beta-actin2
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80068-plasmid-map-sequence-id-152622

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  18. N-His-GvpC-ssrA

    Plasmid
    #153297
    Purpose
    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d
    Insert
    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    Tags
    His-tag and ssrA tag
    Expression
    Bacterial
    Mutation
    1) N-terminal 6xHis tag flanked by 1x glycine res…
    Promoter
    T7
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153297-plasmid-map-sequence-id-310693

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  20. pEGB3 alpha1 35s:PIF6:tNos (GB0170)

    Plasmid
    #160606
    Purpose
    TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4
    Insert
    PIF6
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    P35S
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160606-plasmid-map-sequence-id-322045

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Showing: 10201 - 10220 of 13850 results