We narrowed to 14,683 results for: NTS;

1. GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

   Plasmid

   #166991

   Purpose

   Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(4), Hairpin = 0 nt
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

   Plasmid

   #166990

   Purpose

   Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(1), Hairpin = 0 nt
   

   Expression

   Mammalian

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

   Plasmid

   #166987

   Purpose

   Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(4), Hairpin = 0 nt
   

   Expression

   Mammalian

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt

   Plasmid

   #166995

   Purpose

   Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination module:PolyU(0), Hairpin = 0 nt
   

   Expression

   Mammalian

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-NtXNtT4aR1AtR2NtB2

   Plasmid

   #160887

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   PT7-Nt-rbcS-T4a
   

   Expression

   Bacterial

   Promoter

   rbcS-T4a: T7, chaperones: T10

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hy_pMtnA UTR delta 21-55 eGFP SV40

   Plasmid

   #132653

   Purpose

   Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   MtnA

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMtnA UTR delta 41-55 eGFP SV40

   Plasmid

   #132652

   Purpose

   Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   MtnA

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0.054

   Plasmid

   #119572

   Purpose

   Level 0 part. Promoter

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   PpetE
   

   Use

   Synthetic Biology

   Mutation

   RBS* added (22 bp) upstream of ATG. Deletion of n…

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMYA5T2/pENTR3C

   Plasmid

   #97409

   Purpose

   CMYA5 (NM_153610, nt 12205 to 12279) T allele (rs10043986) in pENTR3C, Gateway Entry vector

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   CMYA5 (CMYA5 Human)
   

   Use

   Unspecified

   Mutation

   rs10043986 (NM_153610.4:c.12188C>T)

   Available Since

   Aug. 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. EBFP2-Cx30

    Plasmid

    #69022

    Purpose

    Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AC 4-μHOM

    Plasmid

    #113619

    Purpose

    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    4-μHOM variant of EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF13E

    Plasmid

    #42942

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    EN- Human L1 element (L1RE1 Human)
    

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    AgeI-BclI fragment swapped with nt 1927-3708 from…

    Promoter

    CMV MIE, L1 5'UTR

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. msfGFP-Cx43

    Plasmid

    #69024

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-MEK1

    Plasmid

    #68300

    Purpose

    N-terminal MBP tagged human MEK1 gene for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 (MAP2K1 Human, Synthetic)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPEY-GAL-Nat-ADE2

    Plasmid

    #232103

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-Universal.Sth.dCas9

    Plasmid

    #171088

    Purpose

    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    

    Use

    Toxoplasma gondii expression

    Mutation

    Note: A S245F mutation is present but did not app…

    Promoter

    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HP21-TPI-WT-no_introns

    Plasmid

    #131044

    Purpose

    Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-Wildtype-no introns (TPI1 Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

    Plasmid

    #55199

    Purpose

    CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    ECFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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