We narrowed to 17,472 results for: She

1. EF1a_Puro_Telo_v1

   Plasmid

   #195138

   Purpose

   ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Telomere (RTEL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR221 UBR5 ∆UBR

   Plasmid

   #81063

   Purpose

   Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   W1235L (bp g3704t)

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR221 UBR5 ∆HECT

   Plasmid

   #81065

   Purpose

   Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   C2768A (bp t8302g and g8303c)

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB-Tet-On-Arl13b-RhoA sensor

   Plasmid

   #189613

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Arl13b
   

   Use

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Tet3G

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MmEos3.2

   Plasmid

   #203754

   Purpose

   Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Src15 n-term (SRC Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SHC003BSD-EXOC7

   Plasmid

   #133298

   Purpose

   Expresses EXOC7 in mammalian cells

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Insert

   Exocyst complex component 7 (EXOC7 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Syn1-GRAB-HTR6-cilia

   Plasmid

   #189615

   Purpose

   Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6_GRAB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Syn1

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Chr1q_Cassette-Integration_gRNA

   Plasmid

   #195126

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr1q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. plv-AcGFP-SOD1 (G93A)

   Plasmid

   #27142

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Lentiviral

   Tags

   AcGFP

   Expression

   Mutation

   G93A

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-TetOne-Puro-PFDN2

    Plasmid

    #202664

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of PFDN2 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    prefoldin subunit 2 (PFDN2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

    Plasmid

    #216386

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

    Plasmid

    #216387

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pF142 pAcGFP1 SOD1 A4V

    Plasmid

    #26403

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A4V

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pF144 pAcGFP1 SOD1G85R

    Plasmid

    #26405

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G85R

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

16. MSCV-NSD3-short

    Plasmid

    #72552

    Purpose

    expresses 3*FLAG tagged human NSD3-short

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEos3.2FP

    Plasmid

    #203757

    Purpose

    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    NRas c-term (NRAS Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGV3319

    Plasmid

    #170277

    Purpose

    Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    Bst-LF D720A
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    D720A

    Promoter

    T7-lac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PMmEos3.2

    Plasmid

    #203755

    Purpose

    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Lyn n-term (LYN Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OR2M4_Deletion_gRNA

    Plasmid

    #195194

    Purpose

    dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR2M4 dual gRNA (OR2M4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FusionRed-Fibrillarin-7

    Plasmid

    #56118

    Purpose

    Localization: Nucleoli, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pF143 pAcGFP1 SOD1G37R

    Plasmid

    #26404

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G37R

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

24. FusionRed-Caveolin-C-10

    Plasmid

    #56103

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

25. FusionRed-CD36-C-10

    Plasmid

    #56104

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    CD36 (CD36 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

27. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pGL3-DICER-Prom Ebox double mut

    Plasmid

    #25854

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter Ebox double mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-DICER-Prom upstream Ebox Mut

    Plasmid

    #25852

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter upstream Ebox Mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HOXB6 (human) FLAG pCMV

    Plasmid

    #8815

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

32. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pGL3-DICER-Prom downstream Ebox Mut

    Plasmid

    #25853

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Prom downstream Ebox Mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. integrin beta6 777T pBabe puro

    Plasmid

    #13583

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

35. HOXB6 (human) EGFP-fusion

    Plasmid

    #8535

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains eGFP fused to N-terminal of HOXB6 at AA…

    Promoter

    CMV

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

36. integrin alpha9 W999A pcDNA1 neo

    Plasmid

    #13589

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 W999A (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W999A

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pXJ-Nluc-_(PLRSKK)-CA1-19

    Plasmid

    #232657

    Purpose

    Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    CGA (CGA Human)
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-HLA-A0201-ST3

    Plasmid

    #223220

    Purpose

    Expression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteria

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    HLA--A2 heavy chain (HLA-A Human)
    

    Use

    Tags

    Spytag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mEos3.2trLATm2allL+A

    Plasmid

    #203750

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm2(allL+A) (LAT Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. lentiTet-CSK-P2A-mCherry BlastR

    Plasmid

    #186742

    Purpose

    Dox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    CSK (CSK Human)
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge