We narrowed to 1,108 results for: Amot;

1. pMRXIP-GFP-1K8Q

   Plasmid

   #221742

   Purpose

   Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-3K6Q

   Plasmid

   #221741

   Purpose

   Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP-GFP-5K4Q

   Plasmid

   #221740

   Purpose

   Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   C -terminal region of KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-170, mutated K to Q

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-muGFP-hATG2A mLIR

   Plasmid

   #215506

   Purpose

   Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   Autophagy related 2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   muGFP

   Expression

   Mammalian

   Mutation

   P656R (natural variant with no functional relevan…

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

   Plasmid

   #207493

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-851_HA-GD2-28z_HA-TFAP4

   Plasmid

   #207501

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. DHC1-mIAA7 Neo

   Plasmid

   #140543

   Purpose

   DHC1 tagging with mIAA7

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-hSK

   Plasmid

   #27078

   Purpose

   Integration-free (episomal) expression of human SOX2 and KLF4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   SOX2, KLF4 (KLF4, SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1-mVenus-p27K−

   Plasmid

   #176651

   Purpose

   It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

   Depositor

   Mohamed Bentires-Alj

   Article

   Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

   Insert

   mVenus-p27K- (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mutation

   Mutant K- (lacks affinity to Cdk)

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-sumo-h-cGAS

    Plasmid

    #127161

    Purpose

    expression of human cGAS protein

    Depositor

    Thomas Tuschl

    Article

    Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

    Insert

    MB21D1 (CGAS Human)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-mFgf21pro1-luc

    Plasmid

    #101797

    Purpose

    Reporter for mouse Fgf21 promoter activity

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    Fibroblast growth factor 21 promoter (Fgf21 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB multi-Hyg Human collagen type IV alpha 4 chain (COL4A4)

    Plasmid

    #229771

    Purpose

    3xFlag-tagged human Col4A4 to be used with N- or C-tagged LgBiT Col4A5 and SmBiT Col4A3 in Nanobit assay system

    Depositor

    Hirofumi Kai

    Article

    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

    Insert

    Human collagen type IV alpha 4 chain (COL4A4) (COL4A4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-sumo-h-cGASCD(K427E/K428E)

    Plasmid

    #127162

    Purpose

    expression of truncated human cGAS protein

    Depositor

    Thomas Tuschl

    Article

    Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

    Insert

    MB21D1 (CGAS Human)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

    Plasmid

    #228902

    Purpose

    For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

    Depositor

    Bryan Roth

    Article

    Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

    Insert

    hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
    

    Use

    AAV and Synthetic Biology

    Tags

    HA and P2A mCitrine

    Expression

    Mammalian

    Mutation

    R108K

    Promoter

    Chicken Beta Actin

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEFCas9

    Plasmid

    #87115

    Purpose

    SpCas9 expression AAV backbone plasmid under control of nEF promoter

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    nEF-Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIG-702_CD19-BBz_CAR_tNGFR

    Plasmid

    #207485

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, CD19-BBz_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIG-727_HA-GD2-28z_CAR_RFP-tNGFR

    Plasmid

    #207487

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-704_CD19-28z_CAR_tNGFR

    Plasmid

    #207486

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, CD19-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

    Plasmid

    #128327

    Purpose

    Reporter to evaluate YAP1/TEAD-mediated gene transcription

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Inserts

    H2B mCherry
    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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