We narrowed to 62,774 results for: promoter
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His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
TagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209653PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVMutationA95A.A119LPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFH2.2_mCherry-MS2x12
Plasmid#205521PurposeExpress mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
Tags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ApoE2
Plasmid#197146PurposeExpresses ApoE2 in Mammalian cellsDepositorAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_XBB
Plasmid#194494Purposeexpressing SARS-CoV-2 XBB spike protein for pseudovirus productionDepositorInsertSpike (XBB) (S SARS-CoV-2)
ExpressionMammalianMutationV83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…PromoterCMVAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-G-Flamp2-mut
Plasmid#192783Purposecontrol probe of G-Flamp2, insensitive to cAMPDepositorInsertG-Flamp2-mut
TagsHis tagExpressionMammalianPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-SOX2-17-p2a-KLF4
Plasmid#193290Purposeepisomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversionDepositorExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ExRai-AMPKAR(T/A)
Plasmid#192447PurposeNon-phosphorylatable negative-control mutant of ExRai-AMPKAR.DepositorInsertExRai-AMPKAR(T/A)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationThr 6 in AMPK substrate mutated to Ala.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mhYFP
Plasmid#186533PurposeMonomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertMonomeric hyperfolder YFP
ExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2
Plasmid#191566PurposeExpresses an shRNA and mTagBFP2DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)
Plasmid#185914PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-gp32-H6
Plasmid#163912PurposeExpresses T4 gp32 for bacterial expression and affinity purificationDepositorInsertgp32
TagsHisExpressionBacterialPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
Plasmid#129632PurposeVisualization of Akt and ERK activityDepositorInsertPB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
ExpressionMammalianPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 3000
Plasmid#163116PurposeRed fluorescent protein-based glucose sensor for mammalian cells with lower affinityDepositorInsertRed Glifon 3000
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.3_CoV1_D28
Plasmid#170447PurposeExpress SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.DepositorInsertSARS-CoV-1 spike D28 (S SARS-CoV-1)
TagsNAExpressionMammalianMutationlast 28aa deletion in c-terminal tailPromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
