We narrowed to 11,008 results for: T7

Showing: 10441 - 10460 of 11008 results

Results per page:

Sort results by:

CSK22_HUMAN_D0

Plasmid

#79704

Purpose

This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK22 (CSNK2A2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
csd6_4-330/pET28b(+) N-tag

Plasmid

#71351

Purpose

csd6, construct:4-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330/pET28b(+) N-tag

Plasmid

#71352

Purpose

csd6, construct:13-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
csd6_1-330/pET28b(+) N-tag

Plasmid

#71350

Purpose

csd6, construct:1-330, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 )

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (PPP)

Plasmid

#62067

Purpose

Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A, P27A

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pBSWHhc-Lam

Plasmid

#133902

Purpose

Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

Plasmid

#111398

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

Plasmid

#111399

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

Plasmid

#111400

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-ALDH1A1

Plasmid

#189749

Purpose

The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-32a(+)-TGFB1

Plasmid

#120361

Purpose

Produce human recombinant TGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β1 (TGFB1 Synthetic, Human)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Article

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet_huCAD_ICADL (TevS)

Plasmid

#100098

Purpose

dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

DNA fragmentation factor subunit alpha (CAD, DFFA Human)

Expression

Bacterial

Mutation

two caspase cleavage sites (DETD-117 and DAVD-224…

Promoter

T7

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Article

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ136

Plasmid

#162665

Purpose

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

Depositor

Gregg Beckham

Article

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Insert

MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)

Tags

His

Expression

Bacterial

Mutation

codon optimized for expression in E. coli K12

Promoter

T7/lac

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge