We narrowed to 23,148 results for: REP

Showing: 10461 - 10480 of 23148 results

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pCMV-dCas9-mD3A

Plasmid

#78257

Purpose

Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Mutation

E756A

Promoter

pCMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-V96 ELP

Plasmid

#67853

Purpose

GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

Depositor

Andrew MacKay

Article

Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

Insert

GFP fused to ELP (VPGVG) repeated 96 times

Tags

GFP fused to N-terminal of ELP

Expression

Mammalian

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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TNF-SBP-mCherry_puromycin

Plasmid

#65285

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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p16-1 hTRF2

Plasmid

#12299

Depositor

Titia de Lange

Article

Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Insert

hTRF2 (TERF2 Human)

Expression

Bacterial

Mutation

A434 deleted; There is a stop codon in the 5'…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pAcGFP-MALAT1M

Plasmid

#120382

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

MALAT1 (MALAT1 Human)

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) HIPK3 Sense

Plasmid

#60634

Purpose

Expresses the human HIPK3 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Homeodomain-interacting protein kinase 3 (HIPK3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-Venus-Emi1 (914)

Plasmid

#39854

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Insert

Emi1 (FBXO5 Human)

Use

In vitro transcription

Tags

Venus

Promoter

T7

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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MM39

Bacterial Strain

#42894

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Bacterial Resistance

Streptomycin

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
modified pcAT7-Glo1

Plasmid

#160996

Purpose

Splicing reporter backbone

Depositor

Brenton Graveley

Article

Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

Insert

hemoglobin subunit beta (HBB Human)

Expression

Mammalian

Mutation

Repeated intron 1 with 40 nucelotide deletion

Promoter

CAG

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCAD29_hIRG1_4-461_pvp008

Plasmid

#124843

Purpose

Expresses human CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 462-end.

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pIDS-RESTwt full-length

Plasmid

#109161

Purpose

Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-i53-DM

Plasmid

#92171

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53-DM

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A, P69L, and L70V mutations and no…

Promoter

CMV

Available Since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC-NMYC TRF2 deltaM

Plasmid

#16068

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

Deleted Myb Domain (aa454-500)

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
Chx10 3kb AP (CC#318)

Plasmid

#15205

Depositor

Connie Cepko

Article

Rowan et al Dev Biol. 2005 May 15. 281(2):240-55.

Insert

Chx10 3kb AP reporter (Vsx2 Mouse)

Expression

Bacterial

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (146-425)

Plasmid

#14966

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 146-425

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
YCpLac111

Plasmid

#53249

Purpose

contains a single replication origin (ARS1) used for plasmid replication assay

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Type

Empty backbone

Use

Unspecified

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only