We narrowed to 13,693 results for: crispr cas9

1. pJK430

   Plasmid

   #155377

   Purpose

   PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A2NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX459-CDKN1A

   Plasmid

   #217457

   Purpose

   For CDKN1A (p21) knockout

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for CDKN1A
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMJ920

   Plasmid

   #42234

   Depositor

   Jennifer Doudna

   Article

   Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GFP, HA epitope, and NLS (nuclear localization si…

   Expression

   Mammalian

   Mutation

   codon-optimized synthetic DNA sequence

   Promoter

   CMV

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL198

   Plasmid

   #180472

   Purpose

   For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

   Depositor

   Peggy Lemaux

   Article

   Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   ZmCas9

   Expression

   Plant

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Expression

   Mammalian

   Mutation

   VQR

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMB1610_pRR-Puro

   Plasmid

   #65853

   Purpose

   Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

   Depositor

   Marc Bühler

   Article

   Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

   Insert

   split puromycin N-acetyltransferase coding sequence
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. px458_Conc2

   Plasmid

   #134449

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px458_Conc3

   Plasmid

   #134450

   Purpose

   Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px459V2.0_Conc3

    Plasmid

    #134458

    Purpose

    Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pECNUS_MP01

    Plasmid

    #194350

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9, FAST-Red
    

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Expression

    Mammalian

    Mutation

    VQR

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPOR1CB0022

    Plasmid

    #117537

    Purpose

    Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (Sp Cas9) - extended stem
    

    Expression

    Plant

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pH-ABE8e-spG

    Plasmid

    #200775

    Purpose

    For plant adenine base editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

    Insert

    nCas9-spG(D10A)-TadA8e
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

    Promoter

    maize Ubiquitin-1, OsU3

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiMPH v2

    Plasmid

    #89308

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Insert

    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGLOW30

    Plasmid

    #172697

    Purpose

    C. elegans mScarlet co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mScarlet
    

    Tags

    mScarlet

    Expression

    Worm

    Promoter

    myo-2

    Available Since

    Sept. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGLOW79

    Plasmid

    #177339

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Worm

    Promoter

    myo-3

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge