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pEGFP-C1-hRabin8 L196A

Plasmid

#26727

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

L196A: GEF domain mutant.

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCIS-krPAM2

Plasmid

#25457

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM2 (Pam Rat)

Expression

Mammalian

Mutation

Exon A deleted (exon A extends from nt 1475 to n…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pEU3-NII-GLICNot-C-His

Plasmid

#140187

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

6xHis and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-retroCHMP3-HA

Plasmid

#154178

Purpose

expresses mouse retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Tyr

Plasmid

#170293

Purpose

A knock-out vector for the mouse tyrosinase.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5myc Cdc42 L61

Plasmid

#15906

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Cdc42 (placental isoform) (CDC42 Human)

Tags

myc

Expression

Mammalian

Mutation

Q61L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-FKBP-empty

Plasmid

#221239

Purpose

Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-4xFKBP scaffold

Use

Retroviral

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5myc Cdc42 N17

Plasmid

#15907

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Cdc42 (placental isoform) (CDC42 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
wt HA-dynamin 1 pUHD10-3

Plasmid

#34688

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1L Human)

Tags

HA (variant)

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pIS1 Cyclin D2 long UTR

Plasmid

#21645

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 long UTR (CCND2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pIHEU_miniSOG2 T2A H3.3A-EGFP

Plasmid

#87411

Purpose

UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Expression

Insect

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pB80-iLID-mCherry-RAB5

Plasmid

#174624

Purpose

Optogenetic coupling to early endosomes (RAB5) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

iLID-mCherry-RAB5 (RAB5A Human, Synthetic)

Tags

iLID-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB5: Met1Del

Promoter

Chicken beta-actin

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂PY)

Plasmid

#24808

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the PY motif: PPPY

Available Since

June 22, 2010

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-MCM6-N-22

Plasmid

#54172

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry_eDHFR_SIM#1

Plasmid

#164646

Purpose

tag SIM with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIM

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-NucGFP

Plasmid

#184696

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pZH519

Plasmid

#102672

Purpose

Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pTRF.1-mCMV-udsVenus

Plasmid

#58518

Purpose

Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

Plasmid

#124198

Purpose

Luciferase reporter driven by the Sox2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Sox2 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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