We narrowed to 27,436 results for: Met

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pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-eGO-Matryoshka-LE-LP

Plasmid

#100017

Purpose

Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

cpEGFP-LSSmOrange

Tags

6x HIS tag

Expression

Bacterial

Mutation

Leu and Glu N-term linker and Leu and Pro C-term …

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Tags

His and S

Expression

Bacterial

Promoter

T7

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pFIV-H1-Puro-hPolß

Plasmid

#18663

Depositor

Robert Sobol

Article

Trivedi et al Molecular Pharmacology 2008;74(2):505-16

Insert

shRNA specific to human DNA polymerase beta (POLB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

none

Available Since

Oct. 24, 2008

Availability

Academic Institutions and Nonprofits only
pLC-EGFP-RIP1

Plasmid

#75163

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIPk1

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIPK1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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VanGlow-RR

Plasmid

#83343

Purpose

for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

Depositor

Cheng-Yu Lee

Article

Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

Type

Empty backbone

Use

Luciferase

Tags

mCherry::Renilla(nls)

Expression

Insect

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pIMVAd39T

Plasmid

#98303

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFlag-Alkbh4

Plasmid

#105240

Purpose

Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Alkbh4 (Alkbh4 Mouse)

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Keasling-2187

Plasmid

#100167

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

MevT and MBIS operon

Expression

Bacterial

Promoter

lacUV5

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDule-IBBN (G2)

Plasmid

#85500

Purpose

Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

Insert

IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32G L65E F108W Q109M D158S R162K

Promoter

lpp (constitutive)

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00476

Plasmid

#39585

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5838 (Dref Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-UCHL5

Plasmid

#31219

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

UCHL5 (UCHL5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-DmrC

Plasmid

#61673

Purpose

Non-fluorescent, truncated kinesin-1 fused to DmrC linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-DmrC (Kif5c Rat)

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-NDC80

Plasmid

#31215

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NDC80 (NDC80 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pBabePuro-DNAJA4CDS

Plasmid

#43799

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

DNAJA4 CDS (DNAJA4 Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only