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pPAP_BGL1

Plasmid

#183735

Purpose

Expresses Aspergillus aculeatus β-glucosidase (BGL1)

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

β-glucosidase (AaBGL1)

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-PpAPOBEC1 R33A

Plasmid

#138345

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1 R33A

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1 R33A

Use

Synthetic Biology

Tags

BP-NLS

Mutation

Change of Arg 33 to Ala.

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(3mut)-mGreenLantern

Plasmid

#219662

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMy-CTCF-GFP

Plasmid

#50574

Purpose

Retroviral vector endoding for mouse CTCF fused to GFP

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHiFiNG-P2A-Puro

Plasmid

#136903

Purpose

Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHiFiNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-ReaChR-P2A-dTomato

Plasmid

#114368

Purpose

expresses a Cre-dependent red light-shifted channelrhodopsin variant

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

ReaChR-P2A-dTomato

Use

AAV

Tags

dTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 1-242

Plasmid

#24391

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-242.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoA

Plasmid

#238241

Purpose

For integration of mCherry_P2AT2A_GFP-nb-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1H2_Hormone-recep

Plasmid

#109827

Purpose

Protein expression and purification of NR1H2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1H2_Hormone-recep (NR1H2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q

Plasmid

#132545

Purpose

Expresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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H1.99.luc

Plasmid

#193663

Purpose

Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated Pol III H1 promoter (H1.99) + 41 nucleotides

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-mC-S

Plasmid

#172427

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

mCherry Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2B

Plasmid

#162001

Purpose

Lentiviral expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2B (PIK3C2B Human)

Use

Lentiviral

Tags

eGFP

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pH6HTC_HNRNPA1-Halo

Plasmid

#175307

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_VNp-mNeongreen_CydAB-mCherry

Plasmid

#182391

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
cydAB-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Article

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Insert

EGFP

Tags

6xHis and farnesylation signal

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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PDZD7-TwinStrepII

Plasmid

#83198

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Article

Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

Insert

PDZD7 (Pdzd7 Mouse)

Tags

Twin Strep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET16b-MYPT1(1-314)

Plasmid

#224893

Purpose

Bacterial expression of MYPT1(1-314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

myosin phosphatase targeting subunit 1 (PPP1R12A )

Expression

Bacterial

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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