We narrowed to 32,291 results for: uros

1. pAAV-TRE-EGFP

   Plasmid

   #89875

   Purpose

   EGFP reporter vector of iTango system for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HA-14-3-3 ϵ

   Plasmid

   #48797

   Purpose

   expresses human myc-14-3-3ϵ in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Chen et al Cell. 2003 May 16;113(4):457-68.

   Insert

   YWHAE 14-3-3 ϵ (YWHAE Human)
   

   Tags

   haemagglutinin

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pB80-2xFKBP-L-mCherry-CAAX

   Plasmid

   #193713

   Purpose

   Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   2xFKBP-L-mCherry-CAAX
   

   Tags

   2xFKBP-mCherry

   Expression

   Mammalian

   Mutation

   mCherry: Met1 Deleted

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmPB3-VSV

   Plasmid

   #68039

   Purpose

   Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B3 (Plxnb3 Mouse)
   

   Tags

   VSV

   Expression

   Mammalian

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8e

   Plasmid

   #138489

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-FHC

   Plasmid

   #64874

   Purpose

   lentiviral vector with Flag, HA tags

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. NG-ABE8e

   Plasmid

   #138491

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (NG)
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-jGCaMP7b-XC

   Plasmid

   #182221

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP7b.

   Depositor

   Xiaodong Liu

   Article

   Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

   Insert

   jGCaMP7b-XC
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

   Plasmid

   #222994

   Purpose

   Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-ERP2

    Plasmid

    #175497

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LOC5567066 Gastrin-releasing peptide receptor

    Plasmid

    #115372

    Purpose

    Expresses Ae. aegypti receptor in mammalian cells

    Depositor

    Leslie Vosshall

    Article

    Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

    Insert

    LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9-sgAAVS1-2

    Plasmid

    #129727

    Purpose

    Expressing SpCas9 and sgAAVS1-2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-2
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-GFP/cre

    Plasmid

    #49054

    Purpose

    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

    Depositor

    Fred Gage

    Article

    Tashiro et al Nat Protoc. 2006;1(6):3049-55.

    Insert

    GFP/Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-mCherry-flex-dtA

    Plasmid

    #58536

    Purpose

    Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

    Depositor

    Naoshige Uchida

    Article

    Wu et al Nature. 2014 May 15;509(7500):325-30. do

    Insert

    dtA (DIP_RS18250 )
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-mNeonGreen

    Plasmid

    #99131

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-shBRCA1 #1

    Plasmid

    #44594

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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