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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42,693 results for: LAS

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Showing: 1061 - 1080 of 42693 results

  2. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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  5. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36085-plasmid-map-sequence-id-44618

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  7. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid
    #27055
    Purpose
    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    GFAP
    Available Since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27055-plasmid-map-sequence-id-12796

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  8. pcDNA3-TLR5-CFP

    Plasmid
    #13019
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 5 (TLR5 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13019-plasmid-map-sequence-id-490371

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  9. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

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Showing: 1061 - 1080 of 42693 results