We narrowed to 74,804 results for: LAS

1. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

   Plasmid

   #169737

   Purpose

   Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-scFv-p65-HSF1-Blast

   Plasmid

   #192652

   Purpose

   3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-p65-HSF1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

   Plasmid

   #196722

   Purpose

   Tet-inducible expression of Cas9-T2A-miRFP670nano

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PBS/pU6-HIF1 alpha RNAi plasmid 2

   Plasmid

   #21104

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   RNAi against hypoxia inducible factor 1alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

   Plasmid

   #163044

   Purpose

   CheRiff-eGFP channelrhodopsin (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   CheRiff
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST Halo-flag-CAD

   Plasmid

   #188117

   Purpose

   Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag and Halo tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

   Plasmid

   #66779

   Purpose

   The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

   Depositor

   Thomas Oertner

   Article

   Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

   Inserts

   hbeRhGC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MDH1-miR-221-PGK-GFP-Blast

   Plasmid

   #31519

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-221 (Mir221 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

10. N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast

    Plasmid

    #44245

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    FKBP (FKBP1A Human)
    

    Use

    Lentiviral

    Tags

    Gal4/DBD/aa1-147 and HA

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

    Plasmid

    #71218

    Purpose

    dCas9 fused to the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A(E752A)
    

    Use

    Lentiviral

    Tags

    SPdCas9-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

    Plasmid

    #209781

    Purpose

    Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    cTNT
    

    Use

    AAV

    Tags

    HA and OLLAS

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

    Plasmid

    #209782

    Purpose

    Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    sgRNA
    

    Use

    AAV

    Tags

    HA and OLLAS

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135978

    Purpose

    gRNA against the right loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA2
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    CB

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

    Plasmid

    #135979

    Purpose

    gRNA against the left loxP site of the Ai9 allele

    Depositor

    William Lagor

    Insert

    Ai9 gRNA1
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid

    #101176

    Purpose

    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K848A/K1003A/R1060A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

    Plasmid

    #197687

    Purpose

    plasmid for expressing the DNMT3bCD

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid

    #115142

    Purpose

    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Mb3Cas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-BLAST flag-CAD hCPS1 AD

    Plasmid

    #188130

    Purpose

    Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge