167,966 results

1. pcDNA3-Flag-RNF168

   Plasmid

   #133976

   Purpose

   mammalian expression vector of Flag tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

   Plasmid

   #181843

   Purpose

   GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of residues 12-112 in PKA RIα.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-myc3-NEDD8

   Plasmid

   #19943

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

4. myrAkt delta4-129

   Plasmid

   #10841

   Depositor

   Richard Roth

   Article

   Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

   Insert

   Akt (AKT1 Human)
   

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   Deletion of PH domain (aa4-129)

   Promoter

   SV40

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. HRV-B3 IRES

   Plasmid

   #189969

   Purpose

   Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   HRV-B3 IRES
   

   Use

   Cloning

   Promoter

   N/A

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIH1-puro-control shRNA

   Plasmid

   #26597

   Purpose

   Lentiviral control shRNA vector with puromycin selection.

   Depositor

   Frank Sinicrope

   Article

   Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Available Since

   Dec. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVoPo-GFP

   Plasmid

   #187840

   Purpose

   Constitutive GFP-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   sfGFP
   

   Expression

   Bacterial

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CSF1R-V5/HIS

   Plasmid

   #201984

   Purpose

   expression of human CSF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   CSF1R receptor tyrosine kinase (CSF1R Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBAC-U6-GFP-WPRE

    Plasmid

    #200030

    Purpose

    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. xABEmax

    Plasmid

    #119813

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MGP

    Plasmid

    #26526

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

13. EF1a_MYCN_P2A_Hygro_Barcode

    Plasmid

    #120463

    Purpose

    Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYCN (MYCN Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFP3AA5

    Plasmid

    #185875

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-L1PA1CH_blast rescue

    Plasmid

    #69611

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast rescue cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-RSV-SpCas9

    Plasmid

    #85450

    Purpose

    Express SpCas9 in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pRSV
    

    Use

    AAV

    Promoter

    pRSV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV7.1_3xFLAG-LATS2

    Plasmid

    #172987

    Purpose

    constitutive expression of FLAG-tagged LATS2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-LATS2 (LATS2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-CAG::Cytbow

    Plasmid

    #158995

    Purpose

    Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Cytbow
    

    Tags

    The default expressed EBFP2 is fused to H2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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