We narrowed to 16,814 results for: invs

1. pDONR221 MLK1 D294A

   Plasmid

   #60542

   Purpose

   Donor vector for human MLK1 D294A mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   MLK1 (MAP3K9 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   D294A

   Promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA Flag PGC1 T177A S538A

   Plasmid

   #18093

   Depositor

   Bruce Spiegelman

   Article

   Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

   Insert

   PGC-1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T177A S538A

   Promoter

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO HSPH2

   Plasmid

   #19454

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 4 (HSPA4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations A381T, T732A, and an insertion of "…

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. beta catenin binding mutant of alpha catenin sensor

   Plasmid

   #71711

   Purpose

   This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin Δβ-cat mutant FRET Sensor
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Deleted amino acid residues 48- 163

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dom neg CstF64

   Plasmid

   #127250

   Purpose

   Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   CstF64 delta 282 (CSTF2 Human)
   

   Use

   Other

   Tags

   Expression

   Mammalian

   Mutation

   insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

   Promoter

   pEF1a

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. anti-GFP scFv [N86/38] with sortase tag

   Plasmid

   #204419

   Purpose

   Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

   Insert

   Recombinant mouse scFv targeting GFP (Aequorea victoria)
   

   Use

   Tags

   6xHis tag and Sortase tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TKTL1

   Plasmid

   #72419

   Purpose

   Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis

   Depositor

   David Sidransky

   Article

   Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66

   Insert

   TKTL1 (TKTL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   may have one or two synonymous substitutions

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-PPO-Venus

   Plasmid

   #139502

   Purpose

   Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cer(1-158)-RGS7

   Plasmid

   #55760

   Purpose

   An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   mCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
   

   Use

   Tags

   Cer(1-158) was fused to the amino terminus of RGS…

   Expression

   Mammalian

   Mutation

   RGS7 was amplified via PCR, which added an N-term…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR-HA-IKKalpha

    Plasmid

    #15469

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA6.2EmGFP-cDAXX

    Plasmid

    #169727

    Purpose

    Mammalian expression construct of GFP tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    cDAXX (DAXX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Comprises residues 495-740

    Promoter

    CMV

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. anti-VGluT1 scFv [N28/9] with sortase tag

    Plasmid

    #204424

    Purpose

    Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
    

    Use

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. anti-PSD-95 scFv [K28/43] with sortase tag

    Plasmid

    #204422

    Purpose

    Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting PSD-95 (Homo sapiens)
    

    Use

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Lyso-AktAR2

    Plasmid

    #64933

    Purpose

    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-AktAR2 (LAMP1 Budding Yeast, Human, Synthetic)
    

    Use

    Tags

    Cerulean3, Full-length LAMP1, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCRII-Topo Vglut2 in situ probe

    Plasmid

    #45639

    Depositor

    Jeffrey Macklis

    Article

    Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

    Insert

    Vglut2 in situ probe (Slc17a6 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 2477-2993 of slc17a6 (Vglut2…

    Promoter

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5-GFP-PKHD1

    Plasmid

    #108408

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170328

    Purpose

    Double-floxed inverse Orientation V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO HIS DNAJA2

    Plasmid

    #19546

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge