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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,044 results for: invs

Showing: 10921 - 10940 of 15044 results

  1. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pFUSEss-CHIg-mG1_Arg322Lys

    Plasmid
    #105849
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arg322Lys in constant region of IgG1 heavy chain
    Promoter
    hEF1-HTLV
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105849-plasmid-map-sequence-id-206011

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  6. pDONR P4-P1R-EGFP

    Plasmid
    #48348
    Purpose
    Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    EGFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48348-plasmid-map-sequence-id-70559

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  12. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121816
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121816-plasmid-map-sequence-id-237364

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  16. pKK-TEV-mEGFP

    Plasmid
    #105794
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105794-plasmid-map-sequence-id-211381

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  17. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid
    #123343
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-CKAR
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123343-plasmid-map-sequence-id-307094

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Showing: 10921 - 10940 of 15044 results