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pCMV-TadA-TadA*

Plasmid

#125661

Purpose

Expresses the TadA-TadA* dimer in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

TadA-TadA*

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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GGE014

Plasmid

#120765

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFH32

Plasmid

#125809

Purpose

Level0 SpCas9-NG, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9-NG, human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1658

Plasmid

#119179

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS2

Expression

Bacterial

Promoter

pGAP

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFH34

Plasmid

#126008

Purpose

Level0 Arabidopsis AtU6-26 Pol III promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Arabidopsis AtU6-26 Pol III promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3

Plasmid

#203491

Purpose

jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Gateway entry vector

Tags

His-tag and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GGE257

Plasmid

#120771

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Xpr2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pZSm45 sodA

Plasmid

#48890

Purpose

Lux-regulated expression of sodA enzyme

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

sodA LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18

Plasmid

#79933

Purpose

PH-4GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pH6-mNeon

Plasmid

#178460

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-6HA

Plasmid

#18035

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6 (Wnt6 Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pICSL80001

Plasmid

#50326

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (P. pyralis)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(1.3)-L4

Plasmid

#32577

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 1.3kb version

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pRSETA-betaFlincG

Plasmid

#19736

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

beta-FlincG

Tags

His

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pAGT707

Plasmid

#51835

Purpose

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Tomato Mosaic virus 5'UTR+ CCAT

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-1xSunTag

Plasmid

#67177

Purpose

CRISPaint universal donor for SunTag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

1xSunTag v4

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Cas9m3

Plasmid

#47315

Purpose

Cas9 D10A+D839A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
p3xFLAG-zDrosha

Plasmid

#171540

Purpose

Encodes Zebrafish Drosha protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

drosha (rnasen Zebrafish)

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-5bHA

Plasmid

#17377

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5bHA (Wnt5b Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMON-HsNMT2

Plasmid

#67476

Purpose

Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only