We narrowed to 2,711 results for: FRT

1. pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

   Plasmid

   #189792

   Purpose

   Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (HBB Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ponasterone A inducible

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

   Plasmid

   #171630

   Purpose

   Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

   Depositor

   Christopher A French, Chris French

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   BioTAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF5B-FRT-AP-Smo-YFP-DEST

   Plasmid

   #49099

   Purpose

   Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Smo (Smo Mouse)
   

   Use

   Tags

   YFP and acceptor peptide (AP)

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5/FRT-DEST EVC2-Flag

   Plasmid

   #41005

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA5/FRT/TO_COX8-Halo-PanK

   Plasmid

   #197949

   Purpose

   Expression of FRET_control in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO-Venus-Flag (865)

   Plasmid

   #40998

   Depositor

   Jonathon Pines

   Article

   Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

   Type

   Empty backbone

   Use

   Tags

   3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA5/FRT/TO_CoA-Snifit(V97T)

   Plasmid

   #197944

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-V97T, D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT/TO_CoA-Snifit(G41)

   Plasmid

   #197942

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-GFP-mCherry-3pGW

   Plasmid

   #53965

   Purpose

   Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

   Inserts

   GFP
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

    Plasmid

    #55614

    Purpose

    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    C4762 mutated to serine to render HERC2 catalytic…

    Promoter

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 FRT TO Myc ArA K162R

    Plasmid

    #59805

    Purpose

    Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora A (AURKA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K162R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

    Plasmid

    #86874

    Purpose

    To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    MORF4L1 (MORF4L1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5 FRT-Hygro hTERT FL-Firefly

    Plasmid

    #171057

    Purpose

    hTERT Full-length-expressing splicing minigene

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    hTERT FL-Firefly
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

    Plasmid

    #113549

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A

    Plasmid

    #42044

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. p{Mhc-F4-678-eGFP::FRT,attB}

    Plasmid

    #182185

    Purpose

    fluorescent reporter gene with yeast recombination site within intron

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid

    #59813

    Purpose

    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    K821R

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)

    Plasmid

    #189794

    Purpose

    Express GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

    Plasmid

    #212112

    Purpose

    Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of GABARAP

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

    Plasmid

    #202617

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    FRT-JEDI-1P-Kv
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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