We narrowed to 6,910 results for: dna plasmids

1. pcDNA3.1-H2-18 Heavy chain-SpyTag003

   Plasmid

   #216307

   Purpose

   Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5FRT/TO_HA-mAID-nanobody

   Plasmid

   #117713

   Purpose

   Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   mAID-nanobody
   

   Tags

   triple HA

   Expression

   Mammalian

   Mutation

   codon optimized mAID for murine expression

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.0_nucLAMA-G97 IRES EGFP

   Plasmid

   #136620

   Purpose

   Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-mas-KB-1753-Nluc

   Plasmid

   #158603

   Purpose

   Plasmid expressing KB-1753-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. miR-vector-RFP pCDNA

   Plasmid

   #97143

   Purpose

   for expression of miRNA and TagRFP

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Type

   Empty backbone

   Tags

   TagRFP

   Expression

   Mammalian

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 FRT/TO Plk4 KD-mCherry

   Plasmid

   #80270

   Purpose

   mammalian expression plasmid for kinase dead Plk4

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D154A kinase dead

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.0_nucLAMA-F98 IRES EGFP

   Plasmid

   #136618

   Purpose

   Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 WT MYOC eGLuc2 FLAG

   Plasmid

   #192820

   Purpose

   Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

   Depositor

   John Hulleman

   Article

   Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

   Insert

   myocilin (MYOC Human)
   

   Tags

   FLAG and enhanced Gaussia luciferase (M43I/M110I)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 FRT/TO MycLAP-STIL ∆CC

    Plasmid

    #80268

    Purpose

    mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    missing conserved coiled-coil (amino acids 721-74…

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-IARS2-H112A/P115A

    Plasmid

    #232338

    Purpose

    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Expression

    Mammalian

    Mutation

    Putative isoleucine binding deficiency mutation H…

    Promoter

    CMV

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-IARS2-K664A/K667A

    Plasmid

    #232339

    Purpose

    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Expression

    Mammalian

    Mutation

    Putative tRNA binding deficiency mutation K664A A…

    Promoter

    CMV

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Flag C/EBPa

    Plasmid

    #66978

    Purpose

    Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP alpha (Cebpa Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hACE2-LgBiT

    Plasmid

    #215837

    Purpose

    Expresses hACE2 receptor with LgBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    hACE2-LgBiT (ACE2 )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid

    #207150

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI3 (GNAI3 Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-HSF1Δ∼PRD

    Plasmid

    #71725

    Purpose

    plasmid expressing mic-his tag phosphorylation-deficient hHSF1

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    phosphorylation-deficient HSF1 (HSF1 Human)
    

    Tags

    mac 6xHIS

    Expression

    Mammalian

    Mutation

    15 phosphorylation sites in RD mutated to Alanine

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid

    #207149

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI2 (GNAI2 Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)Q

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-HA-DRE-1-pcDNA3.1-

    Plasmid

    #52513

    Purpose

    expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Dre-1 (dre-1 Nematode)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 (+) -myc-OGA(GS-544)C181

    Plasmid

    #193996

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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