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GST-PRDM16 681-880

Plasmid

#53349

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Publication

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 681-880

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
GST-PRDM16 455-680

Plasmid

#53348

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Publication

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 455-680

Promoter

tac

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
PTPN11

Plasmid

#38965

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPN11 (PTPN11 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLenti-UltraCas12a-6xNLS

Plasmid

#209023

Purpose

Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

UltraCas12a-6xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

M537R/F870L

Promoter

EF-1a

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

Plasmid

#198337

Purpose

PB plasmid expressing Halo-tagged PP7 coat protein

Depositor

Michael Ward

Publication

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

PP7 coat protein 3x Halo-GB1

Use

Piggybac

Tags

HaloTag

Expression

Mammalian

Promoter

Ef1a

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT6_SEPT7-TEV-Strep

Plasmid

#174499

Purpose

bacterial co-expression of human SEPT6 and of human SEPT7

Depositor

Manos Mavrakis

Publication

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN6 (SEPTIN6 Human)
SEPTIN7 (SEPTIN7 Human)

Tags

TEV-Strep

Expression

Bacterial

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-blast

Plasmid

#167822

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Publication

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 D18N BLAST

Plasmid

#164225

Purpose

lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

Depositor

John Maciejowski

Publication

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D18N

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

Plasmid

#132402

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

Plasmid

#132401

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) EPB41L1

Plasmid

#107508

Purpose

Expressing EPB41L1, puromycin resistant

Depositor

Alejandro Gutierrez

Publication

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

EPB41L1 (EPB41L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_AIFM1

Plasmid

#111659

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AIFM1 (AIFM1 Human)

Expression

Mammalian

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-Myc-CRBN-pGK-HYG

Plasmid

#107376

Purpose

Lentiviral expression of myc-CRBN

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A_BAF_L58R

Plasmid

#101775

Purpose

Expresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

L58R mutation

Promoter

EF1a

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Publication

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUB-sSypHer (RbcS-SypHer)

Plasmid

#84732

Purpose

Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

sSypHer

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs2-RAC1 G12V

Plasmid

#86139

Purpose

Retroviral vector expressing RAC1(G12V)-IRES-Bsd-p2A-RFP

Depositor

David Sabatini

Publication

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

RAC1 (RAC1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

G12V

Promoter

LTR

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-FKBP-GEF(TIAM1)

Plasmid

#85156

Purpose

acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Caspase-8 (C360A)-pcw107-V5

Plasmid

#64632

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CASP8 (transcript variant B) (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article