We narrowed to 31,412 results for: PLE

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TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

GFP

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag7

Plasmid

#169329

Purpose

Expression of H2B-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 (H2BC21 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LacZ in pLX304

Plasmid

#42560

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

LacZ

Use

Lentiviral

Tags

2xV5

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBCW

Plasmid

#62658

Purpose

Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

PhiC31

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPathBrick

Plasmid

#65006

Purpose

E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive native promoters

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pALS099 (pGEX-2TP-cTevHis-DEST)

Plasmid

#139787

Purpose

Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Jeannie Lee, Arneet Saltzman

Article

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available Since

April 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-ElonginB

Plasmid

#29504

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin B (ELOB Human)

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
mTOR_1 shRNA

Plasmid

#1855

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

mTOR (MTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pJAT7

Plasmid

#204292

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-DsRed

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFast-hHus1

Plasmid

#222469

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 DOX off puro IKBKG

Plasmid

#241439

Purpose

Expresses DOX-suppressible IKBKG

Depositor

Richard Possemato

Insert

IKBKG (IKBKG Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Partly codon optimized

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Tags

FLAG and GST

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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VSVG-mCherry-RUSH

Plasmid

#203647

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

VSVG

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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p5155

Plasmid

#175391

Purpose

Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

Depositor

Michael Wiles

Article

Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

Type

Empty backbone

Use

Mouse Targeting; Cloning vector, to be used to de…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

Plasmid

#71720

Purpose

Expressing an mRuby-tagged TIR1 in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)

Tags

mRuby

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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