We narrowed to 36,758 results for: Sis;
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Plasmid#126713Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterADH3Available SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-neoR
Plasmid#117048PurposeIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoterDepositorInsertneoR
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 gRNA17 (FWA)
Plasmid#120250PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA-YFP-R5
Plasmid#106257PurposeExpresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertYellow fluorescent protein
TagsAdded silaffin R5 peptide to C terminus of YFP af…ExpressionBacterialPromoterpL-TetOAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
TagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA
Plasmid#71762Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistantDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE
Plasmid#117757PurposeCodon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised CarCBA, Protein CarD, Ferredoxin CarE
ExpressionBacterialPromoterPLac(UV5)Available SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES 946
Plasmid#112644Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertRTG3AD-GSx7-CIB1-SV40NLS (CIB1 Mustard Weed, Synthetic, Budding Yeast)
ExpressionYeastPromoterADH1Available SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
C2 png genomic in Casper
Plasmid#112971PurposeP element vector for transposon insertion of png genomic DNA into Drosophila genomeDepositorInsertpng genomic DNA (png Fly)
UseP insertion vectorAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
C2 M2 png-myc in Casper4
Plasmid#112968PurposeP element vector for transposon insertion of Myc-tagged png into Drosophila genomeDepositorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 T178A R181A R182A R186A (N4A)
Plasmid#111940PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutationDepositorInsertHsh155 T178A R181A R182A R186A (N4A)
ExpressionBacterial and YeastMutationT178A R181A R182A R186AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q773A R775A R776A Q778A (C4A)
Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
ExpressionBacterial and YeastMutationQ773A R775A R776A Q778AAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP
Plasmid#110828PurposeBacterial expression of E.coli DHFR N23PP mutantDepositorInsertecDHFR
ExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTR596Q-myc
Plasmid#107483PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotypeDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only