Addgene

We narrowed to 18,069 results for: Met;

Showing: 2201 - 2240 of 18069 results

Results per page:

Sort results by:

MYC sgRNA5

Plasmid

#100557

Purpose

Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA5

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7-1333-end

Plasmid

#46073

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-1333-end

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7-856-1351

Plasmid

#46072

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-856-1351

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-ST1-A

Plasmid

#48665

Purpose

Bacterial ST1 repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer A/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS295

Plasmid

#34984

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGR-L3S2P00

Plasmid

#46005

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P00 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-15-LexABD-G4MD-QFAD-hsp70

Plasmid

#46124

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexABD-G4MD-QFAD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Map7-1783-end

Plasmid

#46074

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7D1-1783-end

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MYC sgRNA4

Plasmid

#100556

Purpose

Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA4

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-ST1!TB

Plasmid

#48654

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer B

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP684-3

Plasmid

#99489

Purpose

TEV::eGFP::ER::Ollas::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::Ollas::linker::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGR-L3S2P36

Plasmid

#46007

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P36 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SK-YFP-ST1-B

Plasmid

#48662

Purpose

Bacterial ST1 repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer B/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-ST1!TA

Plasmid

#48653

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer A

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASTattB-cytoFLARE1.0-TEV

Plasmid

#234519

Purpose

Transcriptional reporter for detecting calcium transients in Drosophila larvae (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

CaM-V5-TEVp

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
T7-LacO-Halo-STReTCh-RGD

Plasmid

#216532

Purpose

Expresses HaloTag-STReTCh-RGD fusion protein in bacteria

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

HaloTag-STReTCh-RGD

Use

Tags

6xHis and HaloTag

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-2ndVal DsRedEx2

Plasmid

#104024

Purpose

Plasmid encoding 2ndVal DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal DsRed-Express2

Use

Low copy

Tags

Expression

Bacterial

Mutation

DsRed-Express2 with valine inserted in 2nd positi…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP605-1

Plasmid

#99482

Purpose

Myc::TEV::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::TEV::Myc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP640-2

Plasmid

#99487

Purpose

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB649

Plasmid

#60136

Purpose

Firefly luciferase (Promega) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-13-QFBDAD-G4MD257-761-hsp70

Plasmid

#46122

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDAD-G4MD257-761

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-12-QFBDAD-G4MD50-761-hsp70

Plasmid

#46121

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDAD-G4MD50-761

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-Ii_VSVG-SBP-EGFP

Plasmid

#65300

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Ii-Str_SBP-EGFP-Ecadherin

Plasmid

#65288

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH-NanoLuc

Plasmid

#173075

Purpose

Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

Plasmid

#31484

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

EF1s HA NLS Sce(opt) T2A IFP1.4

Use

Lentiviral

Tags

HA and NLS

Expression

Mutation

"Opt" indicates the I-SceI coding seque…

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PARG-c002

Plasmid

#173107

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARG (PARG Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

S448-T976

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct_mScarlet_N1

Plasmid

#85054

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Article

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mScarlet-MYO10

Plasmid

#145179

Purpose

Expresses mScarlet-MYO10 in mammalian cells

Depositor

Johanna Ivaska

Article

Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

Insert

mScarlet-MYO10 (MYO10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF806-reci

Plasmid

#186711

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2201 - 2240 of 18069 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests