We narrowed to 18,211 results for: SHE

1. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_mCherry_2A_HA_2xMS2-VP64

   Plasmid

   #68419

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   CMV

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBad-HisB-GRvT

   Plasmid

   #87363

   Purpose

   Expresses GRvT, a tandem heterodimer green and red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

   Insert

   GRvT
   

   Tags

   His-Tag

   Expression

   Bacterial

   Promoter

   AraBad

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. XW09

   Plasmid

   #65833

   Purpose

   Punc-4c-QS-SL2-mcherry3'UTR-unc-54

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   Neurospora crassa OR74A repressor (qa-1S)
   

   Tags

   mCherry

   Expression

   Worm

   Promoter

   Punc-4c

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.11 promPDE4D3

   Plasmid

   #27302

   Depositor

   David E. Fisher

   Article

   Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

   Insert

   prom PDE4D3 (PDE4D Human)
   

   Use

   Luciferase

   Mutation

   N/A

   Available Since

   Jan. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-R-GECO1.2

   Plasmid

   #113937

   Purpose

   Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   R-GECO1.2
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1-PCYT2

   Plasmid

   #81074

   Purpose

   Expresses PCYT2 in mammalian cells

   Depositor

   Erin O'Shea

   Article

   Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

   Insert

   phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrans_9xD3B_Cluc_Norm

   Plasmid

   #68415

   Purpose

   CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   Cypridina Luciferase-2A-Venus YFP
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   YFP: M153T,V163A,S175G ("Venus") YFP Va…

   Promoter

   minimalCMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ERAP1

    Plasmid

    #39174

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Baculovirus expression

    Tags

    TEV-His10-Flag

    Promoter

    polyhedrin

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. XW08

    Plasmid

    #65832

    Purpose

    Punc-4-QF-SL2-mcherry-3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Punc-4

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-shTRAF2#1

    Plasmid

    #44127

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pWB-TRAF2

    Plasmid

    #44114

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. XW52

    Plasmid

    #65838

    Purpose

    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Czipper-QF-AD-NLS
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Pmig-13

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-shTRAF2#2

    Plasmid

    #44128

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Halo-Sox13

    Plasmid

    #200194

    Purpose

    Expression of full length Sox13

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-Sox13 (Sox13 Mouse)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBad-HisB-GdT

    Plasmid

    #87361

    Purpose

    Expresses GdT, a dimeric green fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GdT
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    AraBad

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_9xD3B_Cluc_Norm

    Plasmid

    #68414

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Lentiviral and Luciferase

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-O-GECO1

    Plasmid

    #113938

    Purpose

    Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    O-GECO1
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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