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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,610 results for: NSI

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Showing: 11201 - 11220 of 12610 results

  1. mRFP-mem

    Plasmid
    #55622
    Purpose
    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    mRFP-Mem
    Tags
    The N-terminal 20 residues of neuromodulin are fu…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55622-plasmid-map-sequence-id-86264

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  5. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  7. pYPQ-SpRY-Act3.0

    Plasmid
    #178957
    Purpose
    It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178957-plasmid-map-sequence-id-354306

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  8. pACDura3

    Plasmid
    #138711
    Purpose
    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
    Depositor
    Víctor de Lorenzo
    Article
    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21
    Insert
    ura3 gene (URA3 Budding Yeast)
    Expression
    Bacterial
    Mutation
    optimized ura3 gene for expression in Escherichia…
    Promoter
    pEM7 promoter
    Available Since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138711-plasmid-map-sequence-id-311288

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  12. NTSR1-DuET

    Plasmid
    #213356
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NTSR1 (NTSR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213356-plasmid-map-sequence-id-430917

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  13. pAAV-CaMKII-STChRger2-TS-EYFP

    Plasmid
    #129393
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Promoter
    CaMKIIa
    Available Since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    129393-plasmid-map-sequence-id-258245

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  14. UTS2R-DuET

    Plasmid
    #213386
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    UTS2R (UTS2R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213386-plasmid-map-sequence-id-430893

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  15. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

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  18. pUBC_HA_PP7-mVenus

    Plasmid
    #111268
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mVenus
    Use
    Synthetic Biology
    Tags
    HA, SV40 NLS, and mVenus
    Expression
    Mammalian
    Mutation
    PP7: C68S, S69G [delta]70-75 (non-aggregating)
    Promoter
    hUBC
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    111268-plasmid-map-sequence-id-212603

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  19. pDule2-IBBN (G2)

    Plasmid
    #85501
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85501-plasmid-map-sequence-id-169858

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  20. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233648-plasmid-map-sequence-id-468211

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Showing: 11201 - 11220 of 12610 results