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Plasmid#234367PurposeVon Willebrand factor fused in tandem to superecliptic pHluorin and inserted into pmRFP1-N1 vector from Addgene (Plasmid No: 54635)/Monitoring of WPB pH, Visualization of WPB exocytosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVon Willebrand factor (VWF Human)
TagspHluorinExpressionMammalianAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pStreptagIIx2 Alfatag 3C InsR EABR
Plasmid#234996PurposeFor production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surfaceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInsR-EABR (INSR Human)
Tags2xStreptag-II, Alfatag, HRV 3C siteExpressionMammalianAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE
Plasmid#234440PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-PDGFR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-PDGFR TM DomainExpressionMammalianPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTT3-ecdCD19-ST3-His_withSUMO
Plasmid#223219PurposeExpression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expressionDepositorInsertextracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
TagsHistag, SUMO, and SpytagExpressionMammalianPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
ICMAB - pET22b T7 EncFtn Mms6
Plasmid#225538PurposeConstruction of intracellular magnetite accumulating bacterial systems.DepositorInsertsIron binding protein
Ferroxidase
Material binding peptite
Iron transporter protein
TagsnoExpressionBacterialPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230058PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
Tags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230059PurposeExpresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
Tags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninA-
Plasmid#229698PurposeTransient expression of GFP-alpha-cateninA- in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
TagsEGFPExpressionMammalianMutationL785A, I792A, V796APromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ptetO::bac::gfp
Plasmid#217882Purposeexpresses the bac-BGC in bacterial cells, produces bacillamide DDepositorInsertsDehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase
ExpressionBacterialPromoterptetOAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218863PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNES-EGFP-C1-flex-PABD-Spo20
Plasmid#220081PurposeSpo20-PABD PA biosensor with a flexible linker to aid in nuclear exportDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpo20 (SPO20 Budding Yeast)
TagsX. leavis map2k1.L(32-44)-EGFP-SGGGSGGSExpressionMammalianMutationamino acids 51-91PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI4-3×FLAG
Plasmid#215512PurposeExpresses FLAG tagged human WIPI4.DepositorInsertWD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)
UseRetroviralTags3×FLAGExpressionMammalianAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218864PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-del.p119K120S–eGFP
Plasmid#211554PurposeA piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationdeletion of amino acids 119K 120SPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKGmDUET-NgBR/hCIT
Plasmid#203173PurposeExpression of human cis-prenyltransferase complex for purificationDepositorTagsStrep-Tag II-TEV and internally 6HIS taggedExpressionMammalianPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-7
Plasmid#199212PurposeLanding Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsaminoglycoside phosphotransferase,
enhanced Green Fluorescence Protein
Red Fluorescence Protein
ExpressionMammalianPromoterCMV and thymidine kinase promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Synthetic, Human)
TagsHaloTagExpressionMammalianMutationK599AAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRP1MX6-ins4
Plasmid#195041PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertTRP1
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1 R44Q-14-mVenusC
Plasmid#191394PurposeR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpcDNA3 Flag-MBD1 R44Q-14-mVenusC
Tags3xFLAGExpressionMammalianMutationR44QPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-BBS3/Arl6 [N302/10R]
Plasmid#188211PurposeMammalian Expression Plasmid of anti-BBS3/Arl6 (Human). Derived from hybridoma N302/10DepositorInsertanti-BBS3/Arl6 (Homo sapiens) recombinant mouse monoclonal antibody (ARL6 Mouse)
ExpressionMammalianPromoterDual CMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only