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pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Expression

Yeast

Promoter

HIS3

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

So Iwata, Simon Newstead

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
SSpB-mCherry-p63DH

Plasmid

#176112

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-SV40-GFP

Plasmid

#65445

Purpose

Expression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ and SV40

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
ins:MCS; cryaa:RFP

Plasmid

#110282

Purpose

A zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Promoter

insulin

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein promoter-less

Plasmid

#118006

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 CS puroR

Plasmid

#46748

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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PB-TetR-tdTomato

Plasmid

#212958

Purpose

piggyBac donor vector containing TetR-tdTomato for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetR-tdTomato

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

Plasmid

#160633

Purpose

TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31-RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-T7-hOsa1

Plasmid

#17986

Depositor

Naoko Tanese

Article

Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

Insert

Osa1 (ARID1A Human)

Tags

T7 epitope

Expression

Mammalian

Mutation

Missing 5' end

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pUF1-dCas9-Sir2a

Plasmid

#122510

Purpose

Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Insert

dCas9-Sir2a

Use

CRISPR

Tags

3*FLAG

Mutation

dCas9(D10A,H840A)

Promoter

PfHsp86

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107318

Purpose

Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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KG#203

Plasmid

#110880

Purpose

Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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CDK4-luciferase full length (2135)

Plasmid

#86656

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

2120 base pairs including up to coding nucleotide…

Promoter

CDK4

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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cpGFP-Adhiron_flex

Plasmid

#165044

Purpose

Flexible linker control for POLArISact expression in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

cpGFP-Adhiron (connected with a flexible linker)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gDNMT1.Cas9-T2A-GFP

Plasmid

#170362

Purpose

Plasmid used for Crispr/cas9 based disruption of human DNMT1.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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