We narrowed to 59,942 results for: %s

1. hSyn-BiPOLES-mCerulean

   Plasmid

   #154944

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLAG-Egfr(Velvet)

   Plasmid

   #18788

   Depositor

   Bruce Beutler

   Article

   Du et al Genetics. 2004 Jan . 166(1):331-40.

   Insert

   epidermal growth factor receptor (Egfr Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D833G; signal sequence removed (aa1-24)

   Available Since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1-CAT

   Plasmid

   #173943

   Purpose

   Bacterial expression of isolated PARP1 CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-CAG::Palmbow

   Plasmid

   #158993

   Purpose

   Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Palmbow
   

   Tags

   The default expressed EBFP2 is fused to H2B; oth…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mRFP-human APPL1

   Plasmid

   #22202

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
   

   Tags

   mRFP

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. nCas9-PPE

   Plasmid

   #140445

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINDUCER20_mStrawberry

   Plasmid

   #161734

   Purpose

   Doxycycline-inducible expression of mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Expression

   Mammalian

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET26b_AT118-L-V5-His

   Plasmid

   #222848

   Purpose

   E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Tags

   V5-His

   Expression

   Bacterial

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-egfp-U3-Osp.gRNA

    Plasmid

    #166034

    Purpose

    Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hsyn-GRAB_Ado1.0med

    Plasmid

    #153286

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSIIpuro-TGFa-ScNeo

    Plasmid

    #209895

    Purpose

    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TGFa-ScNeo (TGFA Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-N-CretrcintG

    Plasmid

    #69570

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Has Service

    AAV1

    Insert

    kozak consensus - N-CretrcintG
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-mDyn1 pcDNA3

    Plasmid

    #36263

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 1 (Dnm1 Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TetO-FUW-NfiA

    Plasmid

    #64901

    Purpose

    Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    Nfia (Nfia Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-FLAG-TAPBPR

    Plasmid

    #135500

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Tags

    Luminal FLAG epitope tag and Signal peptide from …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-C-CreintG

    Plasmid

    #69571

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Has Service

    AAV1

    Insert

    kozak consensus - C-CreintG
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-TetO-Irf7

    Plasmid

    #178445

    Purpose

    doxycycline-inducible expression of Mouse Irf7 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Irf7 (Irf7 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-Neo-PTPRT (WT)

    Plasmid

    #16630

    Depositor

    Bert Vogelstein

    Article

    Wang et al Science. 2004 May 21. 304(5674):1164-6.

    Insert

    PTPRT (PTPRT Human)
    

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pLenti6-DEST PINK1-V5 KD

    Plasmid

    #13319

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Kinase dead: K219A/D362A/D384A

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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