We narrowed to 23,733 results for: REP
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Plasmid#101681Purpose"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et alDepositorInsertCdc42
Tags6xHis and FRET Antenna (mCerulean-cpVenus)ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta Myb
Plasmid#13253DepositorInsertRepressor Activator Protein 1 delta Myb (TERF2IP Human)
UseRetroviralTagsFLAGMutationcontains hRap1 aa2-131 + 184-399 and the point mu…Available SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta Br delta M
Plasmid#13256DepositorInsertRepressor Activator Protein 1 delta Br delta M (TERF2IP Human)
UseRetroviralTagsFLAGMutationcontains hRap1 aa2-44 + 184-399 and the point mut…Available SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
TagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-Cre
Plasmid#167939Purposehuman CD81-TEV cleavage site-CreDepositorInserthuman CD81 (CD81 Human)
TagsCre recombinase and TEV protease cleavage siteExpressionMammalianPromoterCMVAvailable SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP-HA-DAT
Plasmid#90244PurposeExpresses YFP-HA-DAT in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
ExpressionMammalianMutationresidues 193–203 of human DAT were replaced in YF…PromoterCMVAvailable SinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-TPX2(1-43)
Plasmid#69107PurposeFor bacterial expression of human TPX2(1-43aa) tagged with GST.DepositorInsertTargeting Protein for Xklp2 (TPX2 Human)
TagsGSTExpressionBacterialMutationAmino acids 1-43 expressed.Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-HRAS
Plasmid#52730Purposeretroviral expression of human HRASDepositorAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPing
Plasmid#47100PurposeExpresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.DepositorInsertsPing cDNA
mPing
hygromycin resistance gene
UsePlant expressionTagsGFPExpressionYeastPromoterCaMV35s and StUbi3Available SinceSept. 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-kanMX6-Purg1
Plasmid#39349DepositorUseYeast genomic targetingTagskanMX6 module - reverse orientationAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
cmamxA2-GFP
Plasmid#107197PurposeExpresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deletedDepositorInsertcmannexin A2 (ANXA2 Human)
TagsGreen Fluorescent ProteinExpressionMammalianMutationall type II Ca2+ binding sites deleted ( D161A, E…PromoterCMVAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-FW/AA
Plasmid#12293DepositorInsertTLR2 (Tlr2 Mouse)
TagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y, F774A and W775A; signal peptide (aa1…Available SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPB-VPR-dSpCas9[StaPL(TI)]
Plasmid#111508PurposeExpresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertVPR-dSpCas9[StaPL(TI)]
ExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, and S122…PromoterPGKAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-NLRP1 codon-optimized
Plasmid#166833PurposeMammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tagsDepositorAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
hP2X3-cALFA
Plasmid#160499PurposeFor the expression of human P2X3 with ALFA tag at C-terminus in mammalian cellsDepositorInserthuman P2X3 (P2RX3 Human, Synthetic)
TagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationΔN5ΔC33-T13P/S15V/V16IAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(deltaNIC)
Plasmid#110191PurposeExpression of mouse MeCP2 (deltaNIC) in mammalian cellsDepositorInsertMeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
TagsEGFPExpressionMammalianMutationdelta N and C termini. Intervening region replace…PromoterCMVAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-deltaHeat
Plasmid#25068DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains the LRR-ROC-COR-Kinase-WD40 domains; Del…Available SinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only