We narrowed to 59,253 results for: {{{s}}}

1. pLenti-PGK-EGFPc3-Neo

   Plasmid

   #189741

   Purpose

   The construct was used to express GFP in mammalian cells as the control for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA Flag PGC1 T177A S538A

   Plasmid

   #18093

   Depositor

   Bruce Spiegelman

   Article

   Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

   Insert

   PGC-1a (Ppargc1a Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T177A S538A

   Promoter

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

   Plasmid

   #182231

   Purpose

   Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

   Depositor

   Stéphanie Cabantous

   Article

   Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

   Inserts

   ras homolog family member B (RHOB Human)
   rhotekin RHO binding domain (RBD)
   

   Use

   Lentiviral

   Tags

   GFP10 (split-GFP tripartite) and GFP11 (M4)

   Expression

   Mutation

   Promoter

   TRE bidirectional

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-H2-Dd

   Plasmid

   #135518

   Purpose

   Mammalian expression of myc-tagged H2-Dd

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Dd (H2-D1 Mouse)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MEIS1A-MIY

   Plasmid

   #21013

   Depositor

   Corey Largman

   Article

   Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

   Insert

   MEIS1A (Meis1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS-hPhyB621-mCherry-HRasCT

   Plasmid

   #100282

   Purpose

   Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome B (1-621 aa) (PHYB Mustard Weed)
   

   Use

   Tags

   mCherry fused with HRas C-terminus for plasma mem…

   Expression

   Mammalian

   Mutation

   Human codon optimized PhyB (1-621 aa)

   Promoter

   CAG promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Ost4-GFP-MTn

   Plasmid

   #154351

   Purpose

   Mammalian expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ePB-PURO-FLAG-METTL3-APPA

   Plasmid

   #160253

   Purpose

   For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   tight TRE promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1859

   Plasmid

   #205972

   Purpose

   FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS1860

    Plasmid

    #205973

    Purpose

    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid

    #165490

    Purpose

    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Glyco-Venus-bPAC(S27A)-Myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX5X.1 PINK1 KD

    Plasmid

    #13322

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496; Kinase dead: K219D/D362A/D3…

    Promoter

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

14. px330 Rosa26 Gu1 (LH416)

    Plasmid

    #99629

    Purpose

    Plasmid used to target the wildtype Rosa26 allele with Cas9

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-shSmad2

    Plasmid

    #37051

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-Mito-AcGFP-MTn

    Plasmid

    #154350

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FpG5.BFP.Reporter

    Plasmid

    #134967

    Purpose

    Lentiviral vector for enhancer reporter assay

    Depositor

    Beat Bornhauser

    Article

    Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mini Fgf4 promoter

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.E2.ChETA

    Plasmid

    #220310

    Purpose

    Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

    Depositor

    Matt Rowan

    Article

    Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

    Insert

    ChETA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    S5E2

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. KG#205

    Plasmid

    #110881

    Purpose

    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

    Inserts

    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Asp 448 to Asn

    Promoter

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-SI-112

    Plasmid

    #131127

    Purpose

    C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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