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pUC118-FLIP-Puro

Plasmid

#84538

Purpose

a mock vector for generating FLIP targeting vectors

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

FLIP - Puro

Use

Cre/Lox and Mouse Targeting

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pJT119b

Plasmid

#50551

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAG1242 NLS-mCherry-FIREtag

Plasmid

#205170

Purpose

Expresses NLS-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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EC67895

Plasmid

#209506

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

6xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMU-TGNVHAr

Plasmid

#61178

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-pA

Plasmid

#210583

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_low

Plasmid

#128851

Purpose

Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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EC70208

Plasmid

#209469

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70198

Plasmid

#209459

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70200

Plasmid

#209461

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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zbFGF

Plasmid

#12309

Depositor

James Thomson

Article

Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

Insert

Fgf2 (fgf2 Zebrafish)

Expression

Bacterial

Mutation

Ala 11 mutated to Pro, Ser 55 mutated to Asn

Available Since

Sept. 25, 2006

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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EC70207

Plasmid

#209468

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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