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pcDNA3.1-mPCSK9

Plasmid

#122678

Purpose

Expresses mouse PCSK9 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9) (Pcsk9 )

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pmyc-TNRC6B

Plasmid

#10978

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

TNRC6B (TNRC6B Human)

Tags

myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pEB1-moxCerulean3

Plasmid

#103975

Purpose

Plasmid encoding moxCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4Mx2

Plasmid

#202681

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSLHDR03_mCherry_tCD19_unique guide

Plasmid

#124062

Purpose

Construction of 1st donor for scarless genome editing with unique guide

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

mCherry
tCD19

Use

Mouse Targeting; Human targeting

Promoter

Human UbC modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCE38

Plasmid

#174432

Purpose

C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCE41

Plasmid

#174433

Purpose

C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)

Plasmid

#199165

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

Plasmid

#192828

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4C

Plasmid

#202680

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidC (608-773) (sidC Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ gfp-zASC

Plasmid

#98703

Purpose

GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription

Depositor

Anna Huttenlocher

Article

Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604.

Insert

Apoptosis associated speck like protein containing CARD (pycard Zebrafish)

Use

Zebrafish/xenopus expression

Tags

GFP

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuroATT

Plasmid

#47539

Purpose

Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

Depositor

David Weinstock

Article

Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only
pNkx-GFP

Plasmid

#45497

Depositor

Sean Wu

Article

Wu et al Cell. 2006 Dec 15;127(6):1137-50. Epub 2

Insert

2.5kb mouse Nkx2.5 enhancer and promoter fragment (Nkx2-5 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

2.5kb Nkx2.5 enhancer and promoter fragment

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRrsGreenF-E

Plasmid

#205764

Purpose

Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

JNK KTR rsGreenF-E

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTP341 pHAGE2 CMV TagBFP-3xFLAG

Plasmid

#199391

Purpose

Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

TagBFP-3xFLAG

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgAAVS1-HepmCherry

Plasmid

#192827

Purpose

Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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