We narrowed to 19,577 results for: Mut
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Plasmid#139163PurposeSB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1) CCS-CTS deletion mutant; the other side contains RFP and puromycin resistance genes.DepositorInsertcaffeine synthase 1 [Coffea arabica] CCS-CTS region deletion
UseTransposonExpressionMammalianMutationdeleted amino acids 304-319PromoterEF1αAvailabilityAcademic Institutions and Nonprofits only -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
TagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag-EIF4E-TM
Plasmid#212658PurposepiggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)DepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsEIF4E (W56L, W102L, W166L)ExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R1R2
Plasmid#141056PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-I997A
Plasmid#111828PurposeVinculin tension sensor with point mutation affecting ability to bind actinDepositorInsertVinculinTS (I997A)
ExpressionMammalianMutationchanged isoleucine 997 to alaninePromoterCMVAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-mGold
Plasmid#157996PurposeExpression of mGold in mammalian cellsDepositorInsertmGold
ExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterActin promoter with CMV enhancerAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_5
Plasmid#60566PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_5
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_7
Plasmid#60567PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_7
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
Plasmid#50460PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Bt
Plasmid#112497PurposeTransposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).DepositorInsertsINSeq-adapted mariner transposon
Himar1C9
Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM4D(Gi)-mCherry
Plasmid#50461PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsHA and mCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
ExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45AAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-Rncp-iGluSnFR1
Plasmid#107336PurposeRed fluorescent glutamate sensor with ncp topology. Membrane-displayedDepositorInsertRncp-iGluSnFR1
ExpressionMammalianPromoterCMVAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
BFP dest clone
Plasmid#71825PurposeLentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
BFP-Rab5CA (Q79L)
Plasmid#205705PurposeMammalian expression of BFP-tagged mutant Rab5CADepositorAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only