We narrowed to 2,901 results for: BFP

1. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

   Plasmid

   #193968

   Purpose

   Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   hPol eta (POLH Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

   Plasmid

   #214995

   Purpose

   Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR hEF1A-BFP-P2A-rtTA-WPRE

   Plasmid

   #223288

   Purpose

   A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   BFP-P2A-rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

   Plasmid

   #236246

   Purpose

   Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   U6 and human Synapsin1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

   Plasmid

   #102244

   Purpose

   Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

   Depositor

   Jacob Corn

   Insert

   dCas9-HA-BFP-KRAB-NLS
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-TagBFP-KRAB-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211462

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #72667

   Purpose

   Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

   Plasmid

   #191146

   Purpose

   Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   Bdnf.t1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-AID-Ns3h-UGI-T2A-mTagBFP2

   Plasmid

   #221223

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-Ns3h-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

    Plasmid

    #226009

    Purpose

    DjCas13d guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid

    #202824

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0208 CMV-TO-BFPmut-stop-t70587 (FLP-IN)

    Plasmid

    #191138

    Purpose

    Expression of Trigger 1 in 3' UTR of an inactive BFP in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t70587
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti

    Plasmid

    #115797

    Purpose

    Expresses mTagBFP in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mTagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

    Plasmid

    #205160

    Purpose

    express β-ACTIN-3′UTR-8xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211463

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-dead
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    TRE

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge