We narrowed to 13,904 results for: RAP

1. pL106P-LUC-ENTR1A(w451-1)

   Plasmid

   #17455

   Purpose

   Gateway entry vector, Degron N-term Luciferase

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   FKBP-Luciferase fusion (MTOR firefly)
   

   Use

   Luciferase; Entry vector

   Tags

   FKBP12DD

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYG205

   Plasmid

   #200836

   Purpose

   Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives

   Depositor

   Boris Görke

   Article

   Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

   Insert

   rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P-BAD

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-PHB-8

   Plasmid

   #122233

   Purpose

   Expresses shRNA targeting the coding sequence of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-PHB-4

   Plasmid

   #122231

   Purpose

   Expresses shRNA targeting the 3' UTR of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   PHB (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO-PHB-6

   Plasmid

   #122232

   Purpose

   Expresses shRNA targeting the coding sequence of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUDP123

   Plasmid

   #107269

   Purpose

   pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23 C2_8

   Plasmid

   #60286

   Purpose

   This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   RAP1A inactive enhancer (RAP1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pENTR D-TOPO-C2_8

   Plasmid

   #60249

   Purpose

   This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   RAP1A inactive enhancer (RAP1A Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-FHH-MTORKinaseDomain

    Plasmid

    #120708

    Purpose

    Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MTOR Kinase Domain (MTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 1967-2549

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-FHH-MTOR HEAT

    Plasmid

    #120709

    Purpose

    Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MTOR HEAT 1-9 (MTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 1-409

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

15. KHBD00065

    Plasmid

    #34224

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG10318 (NC2alpha Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Q54A

    Plasmid

    #217912

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP Q54A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Glutamine at position 55 and 267 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. T40A

    Plasmid

    #217911

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP T40A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Threonine at position 41 and 253 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJM166B

    Plasmid

    #218123

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19_Ntag_BbsI-Mammalian

    Plasmid

    #186674

    Purpose

    N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Mammalian N-terminal Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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