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pMN-009

Plasmid

#195413

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

Plasmid

#216664

Purpose

Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

Depositor

Leonardo Morsut

Inserts

ETV2 (Etv2 Mouse)
BFP
HygromycinR

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGAN230

Plasmid

#131122

Purpose

reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-CoChR-mRuby2-ST

Plasmid

#109131

Purpose

Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CoChR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bax1-2/L-6

Plasmid

#30533

Depositor

Richard Youle

Article

Edlich et al Cell. 2011 Apr 1. 145(1):104-16.

Insert

BCL2-associated X protein (BAX Human)

Tags

eGFP

Expression

Mammalian

Mutation

F30C E44C C62S L63C C126S P130C

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
GEARBOCS-SPARCL1-N-mCherryTag

Plasmid

#218186

Purpose

To tag Hevin with mCherry at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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P70a-deGFP-sc101-kan

Plasmid

#184841

Purpose

Expression of deGFP

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

promoter OR2-OR1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pQL47 EGFP-VAMP-GST

Plasmid

#122971

Purpose

Reporter of botulinum toxin B cleavage

Depositor

Chandra Tucker

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

EGFP-VAMP3-GST

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gi)

Plasmid

#230008

Purpose

Expresses anti-GFP PAGER(Gi) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM4D

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PCTAIRE-1-HA

Plasmid

#1999

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pSIN REP5-GFP-GluR2 (Q)

Plasmid

#24003

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR2 (Gria2 Rat)

Use

Sindbis virus production

Tags

GFP

Mutation

R607Q

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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3TERM_Nos-35S

Plasmid

#136116

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos-35S (A. tumefaciens, CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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LbdCas12a KRAB

Plasmid

#188503

Purpose

Expresses FLAG tagged LbdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-CD44b-TurboID

Plasmid

#163850

Purpose

In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

CD44b (cd44b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NAIP6

Plasmid

#60202

Purpose

Mouse NAIP6 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP6

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NAIP6 (Naip6 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAIP

Plasmid

#74171

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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