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Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_eCB2.0
Plasmid#164604PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in neuronsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVMutationS383TPromoterhSynAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
m6A-Tracer-NES
Plasmid#159607PurposeConstitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertm6A-Tracer-NES
ExpressionMammalianPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-GFP-MT2A
Plasmid#11613DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmetallothionein 2A (MT2A Human)
TagsGFPExpressionMammalianAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AID-BE3
Plasmid#100803PurposeExpresses AID-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertactivation induced cytidine deaminase (AICDA S. pyogenes, Human)
ExpressionMammalianPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-U6-gRNA-UbC-DsRed-P2A-Bsr
Plasmid#83919PurposeLentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastRDepositorInsertsDsRed-Express2
Bsr
UseCRISPR and LentiviralTagsP2APromoterhUbCAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CARPID dCasRx-BASU
Plasmid#153303Purposeexpress CARPID dCasRx-BASU fusion protein in mammalian cellsDepositorInsertdCasRx-BASU
UseCRISPR and LentiviralExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti_PE7-2A-BSD
Plasmid#234073PurposeFor lentiviral expression of PE7 with blasticidin resistanceDepositorInsertPE7
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-23:TJP1-mEGFP
Plasmid#87429PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTJP1 Homology Arms with mEGFP-linker (TJP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAAV-hSyn-GRAB_g5-HT3.0
Plasmid#208717PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neuronsDepositorHas serviceAAV8InsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#101061PurposeAAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas serviceAAV1InsertCaMPARI2-F391W
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorHas serviceAAV5InsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-iGluSnFR4s-NGR-WPRE
Plasmid#234437PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas serviceAAV1InsertiGluSnFR4s-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1h
Plasmid#140557PurposeExpresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neuronsDepositorHas serviceAAV9InsertRed fluorescent DA sensor GRAB_rDA1h (high affinity)
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-iGluSnFR4f-NGR-WPRE
Plasmid#234438PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas serviceAAV1InsertiGluSnFR4f-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-Volton2-ST-WPRE
Plasmid#172907PurposeAAV-mediated expression of voltage sensor Volton2 under the Syn promoter, Cre-dependent expression; soma localization targeting sequenceDepositorHas serviceAAV1InsertVoltron2-ST
UseAAV and Cre/LoxTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterhSynapsin1Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-flex-iGluSnFR4s-NGR-WPRE
Plasmid#234441PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas serviceAAV1InsertiGluSnFR4s-NGR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only