173,361 results
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Plasmid#68564PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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pAAV-nEF-Con/Fon-ChR2-mCherry
Plasmid#137142PurposeIntersectional viral expression of ChR2-mCherry in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationH134RPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Halo-p58
Plasmid#166896Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTagDepositorInsertp58 (Lman1 Rat)
TagsHaloTagExpressionMammalianMutationL405Q, R419S- please see depositor commentsPromoterCMVAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1_IVT
Plasmid#222340PurposePlasmid for wild type Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized wtBxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianPromoterhuman PGK1Available SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142
Plasmid#69294PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-U-TVA950
Plasmid#115236PurposeLentiviral transfer plasmid for expression of TVA950DepositorInsertTVA950
ExpressionMammalianAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV52 L1L2
Plasmid#52584PurposeExpresses HPV52 L1 and L2 in mammalian cellsDepositorInsertsHPV52 L1
HPV52 L2
ExpressionMammalianAvailable SinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux eGFP
Plasmid#193624PurposeInducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).DepositorInserteGFP
Tags6xHisExpressionBacterialPromoterpLuxAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxR
Plasmid#193625PurposeConstitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.DepositorInsertLuxR
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB504
Plasmid#74846PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
Plasmid#154987PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAXY0001
Plasmid#179834PurposeBinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorInserteYGFPuv
UseBinary vector for plant transformationExpressionPlantPromoter35SAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV MP-135 mRFP
Plasmid#206927PurposeThe micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.DepositorInsertmRFP
UseAAVExpressionMammalianPromoterMP-135Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-RBM24
Plasmid#208052PurposeExpressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cellsDepositorInserthuman RBM24 (ENST00000379052) coding region (RBM24 Human)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)
Viral Prep#104496-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (#104496). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE plasmid DNA. CAG-driven, Cre-dependent jGCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcS-E626-C1C2
Plasmid#200162PurposeThis plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.DepositorInsertsE626
C1C2 domain of lactadherin
TagsHA and HISExpressionMammalianMutationsequence adopted from doi: 10.1371/journal.pone.0…PromoterCMVAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
peYFP-C1-mTOR
Plasmid#73384PurposeExpresses YFP tagged mTORDepositorAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionPromoteralpha-crystallin and cmlc2Available SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only