We narrowed to 17,125 results for: Por

1. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP46-2A(sfGFP)

   Plasmid

   #126990

   Purpose

   Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24c(+)-mtEFTu

   Plasmid

   #30535

   Depositor

   Linda Spremulli

   Article

   Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

   Insert

   Mature bovine mitochondrial elongation factor Tu
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mature form amino acids 44-452. Mito import seque…

   Promoter

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-pnGFP

   Plasmid

   #64913

   Purpose

   Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

   Depositor

   Huiwang Ai

   Article

   Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

   Insert

   pnGFP
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Chromophore tyrosine of the protein mutated to TA…

   Promoter

   CMV

   Available Since

   June 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP28-1A(sfGFP)

   Plasmid

   #126976

   Purpose

   Level 1 vector. sfGFP reporter. Origin pUC (high copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDJSVT91

   Plasmid

   #109305

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   SufI 1-30, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJKR-L-cdaR

   Plasmid

   #62558

   Purpose

   Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP S2TAG

   Plasmid

   #68296

   Purpose

   S2TAG GFP reporter

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   GFP S2TAG
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hSRF-3xHA-IRES-hrGFP (#479)

   Plasmid

   #78347

   Purpose

   Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

   Depositor

   Louis Kunkel, Matthew Alexander

   Insert

   SRF (SRF Human)
   

   Use

   Tags

   3x HA tag and IRES-hrGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pJKR-L-acuR

    Plasmid

    #62568

    Purpose

    Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MARS

    Plasmid

    #205232

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    

    Use

    Tags

    Nuclear Export Sequence and mScarlet-i

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQDKN0248

    Plasmid

    #162313

    Purpose

    Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-sfGFP-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJKR-L-mphR

    Plasmid

    #62560

    Purpose

    Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJUMP26-1A(sfGFP)

    Plasmid

    #126973

    Purpose

    Level 1 vector. sfGFP reporter. Origin p15A (medium copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-hsGFP

    Plasmid

    #64911

    Purpose

    Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

    Depositor

    Huiwang Ai

    Article

    Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

    Insert

    hsGFP
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Chromophore tyrosine of the protein mutated to TA…

    Promoter

    araBAD

    Available Since

    May 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMiR-HPV16-E7

    Plasmid

    #53696

    Purpose

    Luciferase reporter assay for microRNA binding on HPV16 E7

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    HPV16-E7
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA TfR1-miniE

    Plasmid

    #181987

    Purpose

    MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    TfR1-MiniE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIplac211-VPS8-mCherry2Bx6

    Plasmid

    #105273

    Purpose

    Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Vps8 portion (VPS8 Budding Yeast)
    

    Use

    Tags

    mCherry2Bx6

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-luc-miR-122

    Plasmid

    #171557

    Purpose

    a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

    Depositor

    Yan Zeng

    Article

    Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

    Insert

    a sequences perfectly complementary to miR-122
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pG6(5'Pro) (P#1477)

    Plasmid

    #14659

    Depositor

    Matija Peterlin

    Article

    Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

    Insert

    UAS repeats
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only