We narrowed to 13,592 results for: crispr cas9

1. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

   Plasmid

   #105234

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

   Plasmid

   #64113

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

   Plasmid

   #86986

   Purpose

   For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Cas9-venus
   

   Use

   Tags

   Cas9-Venus fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

   Plasmid

   #64112

   Purpose

   Expression of St1 dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1_Cas9-Ck4

   Plasmid

   #136135

   Purpose

   L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:Cas9_3tNos-35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCas9-PBE

   Plasmid

   #98164

   Purpose

   Expresses CRISPR base editor in protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW SpCas9

   Plasmid

   #131506

   Purpose

   lentiviral expression of SpCas9 under hUBC promotor

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   SpCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-nCas9-PBE

    Plasmid

    #98160

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. VP64-dCas9

    Plasmid

    #177171

    Purpose

    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    VP64-dCas9(D10A, H840A)
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xFLag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH-dCas9-PBE

    Plasmid

    #98162

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Rice

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB-dCas9-PBE

    Plasmid

    #98159

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-PBE

    Plasmid

    #98161

    Purpose

    Expresses CRISPR base editor in protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAtCas9-D10A_2

    Plasmid

    #91160

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTaCas9-D10A_5

    Plasmid

    #91170

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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