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pcDNA-D2s-L-V1

Plasmid

#19967

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-N terminal Venus (DRD2 Aequorea Victoria, Human)

Tags

Flag and N terminal Venus

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGWKS10

Plasmid

#139417

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Clover

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/5'USS-GFP

Plasmid

#197884

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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DMP1-GFPtopaz

Plasmid

#114270

Purpose

GFP labeling of mature osteoblasts and osteocytes.

Depositor

David Rowe

Article

Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

Insert

Dmp1 + GFPtopaz (Dmp1 Aequorea victoria, Mouse)

Expression

Mammalian

Promoter

Dmp1

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGWKS5

Plasmid

#139412

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Citrine

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-ECFP

Plasmid

#48353

Purpose

Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

ECFP

Use

Gateway entry vector

Mutation

Contains a stop codon.

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR P4-P1R-EYFP

Plasmid

#48350

Purpose

Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EYFP

Use

Gateway entry vector

Mutation

Contains a Kozak sequence. Does not contain a sto…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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LifeAct-mAvicFP1

Plasmid

#129512

Purpose

Expresses LifeAct-mAvicFP1 in mammalian cells

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

LifeAct-mAvicFP1

Expression

Mammalian

Mutation

mAvicFP1 is AvicFP1 F64L + A206K

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V2

Plasmid

#19968

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-C terminal Venus (DRD2 Aequorea Victoria, Human)

Tags

C terminal Venus and Flag

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
CytERM-mAvicFP1

Plasmid

#129510

Purpose

Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

CytERM-mAvicFP1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p1.1-eGFP

Plasmid

#162738

Purpose

Fluorescent reporter for CHO cells expression studies

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Insert

enhanced green fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCI.neo-rPAM1-M314I

Plasmid

#145802

Purpose

expresses catalytically inactive rat PAM-1 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Expression

Mammalian

Mutation

changed methionine 314 to isoleucine to inactivat…

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_GFP 150TAG

Plasmid

#140017

Purpose

Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSC-NF8-d1-eGFP

Plasmid

#122036

Purpose

AAV vector, NFkappaB-dependent eGFP expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al PLoS One. 2013;8(1):e53156. doi: 10.1371

Insert

eGFP

Use

AAV

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex-Turbo/GFP

Plasmid

#197886

Purpose

Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

Turbo/GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)

Plasmid

#240254

Purpose

Non-attenuated SFV vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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