We narrowed to 2,007 results for: endog

1. qTAG-N-Solo-mNeon-ACTB

   Plasmid

   #207749

   Purpose

   Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+FLAG-KrasnatQ61R

   Plasmid

   #206843

   Purpose

   To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glutamine 61 to Arginine

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJET-22-DExCon-mNeonGreen-R11a

   Plasmid

   #179897

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRepair-mTq2-CTNNB1

   Plasmid

   #153433

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   mTurquoise2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Empty Bridge Control Zfp462-Klf4SE

   Plasmid

   #127666

   Purpose

   Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   gRNAs 129, 135, 115, 117
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PE7-P2A-GFP

   Plasmid

   #222996

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-EGFP
   

   Use

   Tags

   P2A-EGFP, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PE7-P2A-BSD

   Plasmid

   #224122

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-BSD
   

   Use

   Tags

   P2A-BSD, SV40 bpNLS, and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-45b-POLR2A-CTD-mCherry

   Plasmid

   #185011

   Purpose

   Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   POLR2A (Polr2a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid

    #100597

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid

    #224123

    Purpose

    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Q20A_Y23A_Y24F_F35A in La(SSB)1-194

    Promoter

    CMV

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-NUP98a_IDR-EGFP

    Plasmid

    #232766

    Purpose

    Expresses dCas9-NUP98a_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98a_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJV137

    Plasmid

    #124256

    Purpose

    Lentiviral vector (pRRL) containing Her2-ITD specific T cell receptor

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    T cell receptor (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid

    #129415

    Purpose

    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    RAB18 HDR template (RAB18 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Circular 200,100 with interspersed loops (GAPDH)

    Plasmid

    #180183

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Circular 200,100 (mIDUA)

    Plasmid

    #170123

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Idua Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-OptoSTIM1(Cry2(D387A))

    Plasmid

    #70160

    Purpose

    Light-insensitive mutant of OptoSTIM1

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 200,100 with 8 bp bulge loops (GAPDH)

    Plasmid

    #170121

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJV136

    Plasmid

    #124255

    Purpose

    Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 9

    Depositor

    Stanley Riddell

    Article

    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

    Insert

    T cell receptor (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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