Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 1,247 results for: ncl

Clear keywords
Showing: 1221 - 1240 of 1247 results

  1. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55592-plasmid-map-sequence-id-114863

    Enlarge

  2. PGL4.11-COL1A1double mutants

    Plasmid
    #230918
    Purpose
    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant SMAD and TEAD binding…
    Promoter
    COL1A1 with mutant SMAD and TEAD elements
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230918-plasmid-map-sequence-id-460669

    Enlarge

  3. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid
    #131005
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-FLAG WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    FLAG - 10 total FLAG tags
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131005-plasmid-map-sequence-id-305005

    Enlarge

  6. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid
    #102909
    Purpose
    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102909-plasmid-map-sequence-id-200136

    Enlarge

  10. CC1215

    Bacterial Strain
    #79062
    Purpose
    BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.
    Depositor
    T. J. Kappock
    Bacterial Resistance
    Kanamycin
    Available Since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. Escherichia coli DH10B-MOB

    Bacterial Strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Article
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial Resistance
    60 ug/mL of DAP
    Species
    Escherichia coli
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  16. EXPRESSYALI

    Plasmid Kit
    #1000000245
    Purpose
    The EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.
    Depositor
    Irina Borodina
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only

  17. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.
    Depositor
    Helle Ulrich
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. Yeast GPCR-sensor Toolkit

    Plasmid Kit
    #1000000157
    Purpose
    The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. C. elegans miniMos transgenesis kit

    Plasmid Kit
    #1000000031
    Purpose
    29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.
    Depositor
    Erik Jorgensen
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1221 - 1240 of 1247 results