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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,285 results for: crispr

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Showing: 1261 - 1280 of 25285 results

  3. pDN-Sth1Cas9

    Plasmid
    #221386
    Purpose
    Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB
    Depositor
    Deborah Hung
    Publication
    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sth1 Cas9
    Use
    CRISPR
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221386-plasmid-map-sequence-id-440182

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  4. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Publication
    Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Promoter
    none
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  5. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Publication
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    100596-plasmid-map-sequence-id-207339

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  7. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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  9. Cas9 CFP

    Plasmid
    #155278
    Purpose
    Lentiviral Cas9 expression with CFP marker
    Depositor
    Martin Carroll
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9
    Expression
    Mammalian
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155278-plasmid-map-sequence-id-309981

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  10. pLdCH

    Plasmid
    #84291
    Purpose
    Express gRNA and Cas9 in Leishmania with Hygromycin resistance
    Depositor
    Greg Matlashewski
    Publication
    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA and Cas9
    Use
    CRISPR; Leishmania
    Promoter
    L. donovani ribosome RNA promoter
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    84291-plasmid-map-sequence-id-170879

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  11. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3xFLAG and 126aa domain from HSV-1 UL12 fused to …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  12. Oct4-Cr1

    Plasmid
    #66989
    Purpose
    Expresses gRNA that targets stop codon for OCT4
    Depositor
    Danwei Huangfu
    Publication
    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA for Crispr targeting the OCT4 stop codon
    Expression
    Mammalian
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. cPE

    Plasmid
    #183549
    Purpose
    Expresses compact Prime Editor in mammalian cells
    Depositor
    Wen Xue
    Publication
    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 nickase (H840A), Reverse Transcriptase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183549-plasmid-map-sequence-id-365389

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  18. pMA-SpCas9-g5

    Plasmid
    #80788
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80788-plasmid-map-sequence-id-162408

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  19. pSL1166

    Plasmid
    #129520
    Purpose
    pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation
    Depositor
    Scott Lindner
    Publication
    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    Plasmodium, rodent-infectious species
    Tags
    3XFLAG-NLS
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129520-plasmid-map-sequence-id-256186

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Showing: 1261 - 1280 of 25285 results